Abstract
We previously developed a multiplex real-time PCR assay (Rapid Foodborne Bacterial Screening 24 ver.5, [RFBS24 ver.5]) for simultaneous detection of 24 foodborne bacterial targets. Here, to overcome the discrepancy of the results from RFBS24 ver.5 and bacterial culture methods (BC), we analyzed 246 human clinical samples from 49 gastroenteritis outbreaks using RFBS24 ver.5 and evaluated the correlation between the cycle threshold (CT) value of RFBS24 ver.5 and the BC results. The results showed that the RFBS24 ver.5 was more sensitive than BC for Campylobacter jejuni and Escherichia coli harboring astA or eae, with positive predictive values (PPV) of 45.5-87.0% and a kappa coefficient (KC) of 0.60-0.92, respectively. The CTs were significantly different between BC-positive and -negative samples (p < 0.01). All RFBS24 ver.5-positive samples were BC-positive under the lower confidence interval (CI) limit of 95% or 99% for the CT of the BC-negative samples. We set the 95% or 99% CI lower limit to the determination CT (d-CT) to discriminate for assured BC-positive results (d-CTs: 27.42-30.86), and subsequently the PPVs (94.7%-100.0%) and KCs (0.89-0.95) of the 3 targets were increased. Together, we concluded that the implication of...Continue Reading
References
Jan 1, 1994·Annual Review of Microbiology·B Swaminathan, P Feng
Aug 1, 2000·Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases·H NakayamaK Horikawa
Nov 4, 2000·Journal of Clinical Microbiology·D L WilsonJ E Linz
Jun 24, 2003·Radiology·Harold L Kundel, Marcia Polansky
Nov 8, 2003·Journal of Clinical Microbiology·Hiroshi FukushimaRyotaro Seki
Feb 5, 2005·Applied and Environmental Microbiology·Knut RudiAskild L Holck
Mar 24, 2007·Bioinformatics·Triinu Koressaar, Maido Remm
Apr 24, 2007·Diagnostic Microbiology and Infectious Disease·Yoshio IijimaSeishi Asari
Sep 4, 2009·Journal of Clinical Microbiology·James O'LearyBrigid Lucey
Oct 29, 2009·Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases·Noriko KonishiTakao Tsuji
Sep 24, 2010·Journal of Clinical Microbiology·Richard F de BoerAnna M D Kooistra-Smid
Oct 12, 2010·International Journal of Microbiology·Hiroshi FukushimaKeiji Yamaguchi
Jul 26, 2013·Journal of Clinical Microbiology·Brianna LindsayJames P Nataro
Jan 10, 2014·Journal of Clinical Microbiology·Kristina ElfvingMagnus Lindh
Feb 4, 2014·Applied and Environmental Microbiology·Diana SeinigeCorinna Kehrenberg
Mar 25, 2014·BMC Infectious Diseases·Ingrid ZieglerKristoffer Strålin
Jun 4, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J-C KabayizaM Lindh
Jun 26, 2014·Microbiology and Immunology·Kenitiro ItoOrn-Anong Ratchtrachenchai
Jul 15, 2015·Japanese Journal of Infectious Diseases·Jun KawaseKomei Shirabe
Jul 18, 2015·PloS One·Shelly BolotinNatasha S Crowcroft
Citations
May 20, 2020·American Journal of Physical Medicine & Rehabilitation·Min Cheol ChangDonghwi Park
Jan 1, 2021·PloS One·Jui ChoudhuriJames Szymanski
Jan 2, 2021·Viruses·Bruna Aparecida Souza MachadoRoberto Badaró
Jul 6, 2021·Frontiers in Veterinary Science·Hiroshi AsakuraSatoshi Murakami
Aug 3, 2021·Medical Journal, Armed Forces India·Lancelot Mark PintoCamilla Rodriques
Nov 3, 2021·Foodborne Pathogens and Disease·Takuya MizunoYoshihiko Kameyama