Rapid and Accurate Molecular Identification of the Emerging Multidrug-Resistant Pathogen Candida auris

Journal of Clinical Microbiology
Milena KordalewskaDavid S Perlin

Abstract

Candida auris is an emerging multidrug-resistant fungal pathogen causing nosocomial and invasive infections associated with high mortality. C. auris is commonly misidentified as several different yeast species by commercially available phenotypic identification platforms. Thus, there is an urgent need for a reliable diagnostic method. In this paper, we present fast, robust, easy-to-perform and interpret PCR and real-time PCR assays to identify C. auris and related species: Candida duobushaemulonii, Candida haemulonii, and Candida lusitaniae Targeting rDNA region nucleotide sequences, primers specific for C. auris only or C. auris and related species were designed. A panel of 140 clinical fungal isolates was used in both PCR and real-time PCR assays followed by electrophoresis or melting temperature analysis, respectively. The identification results from the assays were 100% concordant with DNA sequencing results. These molecular assays overcome the deficiencies of existing phenotypic tests to identify C. auris and related species.

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Citations

Apr 11, 2018·Antimicrobial Agents and Chemotherapy·Milena KordalewskaDavid S Perlin
Nov 8, 2017·Expert Review of Anti-infective Therapy·Matthew William McCarthy, Thomas J Walsh
Nov 17, 2017·Clinical Microbiology Reviews·Anna Jeffery-SmithColin S Brown
Jun 8, 2018·Current Opinion in Infectious Diseases·Katja SarisAndreas Voss
Nov 24, 2017·Journal of Clinical Microbiology·Emily S Spivak, Kimberly E Hanson
Sep 21, 2018·Journal of Clinical Microbiology·D Joseph SextonAnastasia P Litvintseva
Dec 6, 2018·Acta Clinica Belgica·Klaas DewaeleKatrien Lagrou
Feb 19, 2019·Mycoses·Shabir A Lone, Aijaz Ahmad
May 16, 2019·Microorganisms·Ricardo Franco-DuarteCélia F Rodrigues
Aug 8, 2017·Expert Review of Anti-infective Therapy·Bhagyashri D NavalkelePranatharthi Chandrasekar
Jun 7, 2019·Archives of Pathology & Laboratory Medicine·D Jane HataUNKNOWN College of American Pathologists Microbiology Committee
Jan 31, 2020·Journal of Clinical Microbiology·Brian L Wickes
Oct 3, 2019·MBio·Daniel Murante, Deborah A Hogan
May 19, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ahmed S SultanMary Ann Jabra-Rizk
Apr 17, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Amira ElBaradei
Jul 10, 2020·Pathogens and Disease·Taissa VilaMary Ann Jabra-Rizk
May 1, 2018·Frontiers in Microbiology·Tsun S N KuSamuel A Lee
Aug 8, 2018·Medical Mycology·Kaitlin ForsbergSnigdha Vallabhaneni
Aug 23, 2019·Journal of Fungi·Milena Kordalewska, David S Perlin
Sep 5, 2019·Frontiers in Microbiology·Milena Kordalewska, David S Perlin
May 3, 2019·Current Infectious Disease Reports·Graham M Snyder, Sharon B Wright
Aug 20, 2019·Internal Medicine Journal·Chong W OngUNKNOWN Australian and New Zealand Mycoses Interest Group (ANZMIG); and the Healthcare Infection Control Special Interest Group (HIC
May 23, 2020·Infection and Drug Resistance·Muluneh Ademe, Friehiwot Girma
Nov 7, 2018·Journal of Intensive Care·Andrea CortegianiAnuradha Chowdhary
Oct 9, 2019·Antimicrobial Agents and Chemotherapy·Milena KordalewskaDavid S Perlin
Aug 7, 2019·Expert Review of Molecular Diagnostics·Xin LiPatrick C Y Woo
Jul 8, 2017·Mycoses·Jalila MohsinAbdullah M S Al-Hatmi

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Methods Mentioned

BETA
PCR

Software Mentioned

GraphPad Prism
DNASTAR Lasergene
MxPro

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