PMID: 3748055Oct 1, 1986Paper

Rapid and complete degradation of thymidine by human peripheral blood platelets: implications for genotoxicity assays

Mutation Research
T Shaw, D G MacPhee

Abstract

Small-scale washed cell preparations obtained by Percoll density-gradient fractionation of whole blood were used to study the metabolic fate of [3H]thymidine supplied to isolated human blood mononuclear cells and platelets incubated for up to 24 h in vitro. Two cell fractions were monitored: low molecular weight compounds which were soluble in Triton X-100 and TCA were investigated by thin-layer chromatography, and high molecular weight components, distinguished by their Triton and TCA insolubility, were examined by agarose-gel electrophoresis. Under the conditions used, greater than 99% of added [3H]thymidine was very rapidly degraded. Catabolites were recovered in the Triton-soluble (cytoplasmic) fraction and the extracellular medium. A negligible proportion of added label was associated with Triton- and TCA-insoluble cell fractions. These results confirm and clarify previous data and have important implications for genotoxicity tests which employ in vitro leukocyte cultures.

References

Feb 18, 1976·Biochimica Et Biophysica Acta·G AgamM Djaldetti
Jan 1, 1985·Annual Review of Physiology·H Holmsen
Oct 1, 1983·Archives of Biochemistry and Biophysics·G Soslau

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