Rapid and Dynamic Alternative Splicing Impacts the Arabidopsis Cold Response Transcriptome

The Plant Cell
Cristiane P G CalixtoJohn W S Brown

Abstract

Plants have adapted to tolerate and survive constantly changing environmental conditions by reprogramming gene expression The dynamics of the contribution of alternative splicing (AS) to stress responses are unknown. RNA-sequencing of a time-series of Arabidopsis thaliana plants exposed to cold determines the timing of significant AS changes. This shows a massive and rapid AS response with coincident waves of transcriptional and AS activity occurring in the first few hours of temperature reduction and further AS throughout the cold. In particular, hundreds of genes showed changes in expression due to rapidly occurring AS in response to cold ("early AS" genes); these included numerous novel cold-responsive transcription factors and splicing factors/RNA binding proteins regulated only by AS. The speed and sensitivity to small temperature changes of AS of some of these genes suggest that fine-tuning expression via AS pathways contributes to the thermo-plasticity of expression. Four early AS splicing regulatory genes have been shown previously to be required for freezing tolerance and acclimation; we provide evidence of a fifth gene, U2B"-LIKE Such factors likely drive cascades of AS of downstream genes that, alongside transcriptio...Continue Reading

Citations

Jan 30, 2019·The Journal of Experimental Biology·Timothy M Healy, Patricia M Schulte
Nov 15, 2018·The Plant Journal : for Cell and Molecular Biology·Gongfu YeLianfeng Gu
Jan 22, 2019·The New Phytologist·Yanglin DingShuhua Yang
Feb 23, 2019·Nucleic Acids Research·Ibtissam JabreNaeem H Syed
Apr 9, 2020·G3 : Genes - Genomes - Genetics·Tatsuo KannoMarjori Matzke
Mar 13, 2020·Plant Biotechnology Journal·Sonia BalyanSaloni Mathur
Sep 2, 2020·Biochemical Society Transactions·Paola PunzoGiorgia Batelli
Mar 16, 2019·Frontiers in Plant Science·Rafael CataláJulio Salinas
Oct 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Geeng Loo ChongPaul E Verslues
Jan 11, 2020·Frontiers in Plant Science·Luíza L B DantasCarlos T Hotta
Jun 12, 2020·Journal of Experimental Botany·Vivek Kumar RaxwalManu Agarwal
Dec 19, 2019·Frontiers in Genetics·Paige E PanterAntony N Dodd
Sep 26, 2020·Frontiers in Genetics·Jian ZhangCheng-Guo Duan
Sep 26, 2020·International Journal of Molecular Sciences·Chris J HulattViswanath Kiron
Oct 9, 2018·Frontiers in Plant Science·Michael WisniewskiRajeev Arora
Mar 21, 2019·Frontiers in Plant Science·Cristiane P G CalixtoJohn W S Brown
Feb 11, 2020·Frontiers in Plant Science·Candida NibauNicola Cavallari
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Yongqiang XingLu Cai
Jul 17, 2020·Frontiers in Plant Science·Federico Martinez-SeidelJoachim Kopka
Mar 25, 2019·F1000Research·Daniel Schubert
Jun 4, 2020·The Plant Journal : for Cell and Molecular Biology·Daniel NiyikizaTarek Hewezi
Nov 4, 2020·EMBO Reports·Alexander NeumannMarco Preußner
Nov 3, 2020·The New Phytologist·Ibtissam JabreNaeem H Syed

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.