Rapid and efficient identification of cysteine-rich peptides by random screening of a venom gland cDNA library from the hexathelid spider Macrothele gigas

Toxicon : Official Journal of the International Society on Toxinology
Honoo SatakeG Corzo

Abstract

We identified novel 10 multi-cysteine peptides, namely Magi 7-16, from the spider Macrothele gigas by simple random cDNA screening of the venom gland. Mass analysis of the crude venom detected the mass numbers of the cross-linked forms of all peptides, confirming their presence in the venom. Magi 11, a C-terminus amidated peptide, was chemically synthesized and was indistinguishable from the native peptide proving the feasibility of the method for peptide identification. Moreover, toxicological assays showed diverse lethal or paralytic activities of these peptide toxins on mice and/or insects.

References

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Citations

Feb 24, 2009·Toxicon : Official Journal of the International Society on Toxinology·Diane L Brinkman, James N Burnell
Nov 13, 2008·Toxicon : Official Journal of the International Society on Toxinology·Benjamín Hernández-CampuzanoGabriel Iturriaga
Jul 16, 2008·Toxicon : Official Journal of the International Society on Toxinology·Glenn F KingGraham M Nicholson
May 17, 2008·Toxicon : Official Journal of the International Society on Toxinology·Liping JiangSongping Liang
Feb 28, 2008·Journal of Mass Spectrometry : JMS·P EscoubasG M Nicholson
Sep 20, 2005·Toxicon : Official Journal of the International Society on Toxinology·Sergey Kozlov, Eugene Grishin
Apr 13, 2005·Biochemical and Biophysical Research Communications·Miki HisadaTerumi Nakajima
Jun 12, 2013·Toxicon : Official Journal of the International Society on Toxinology·Fernando Hitomi MatsubaraSilvio Sanches Veiga
May 1, 2012·Toxicon : Official Journal of the International Society on Toxinology·Julie K KlintGlenn F King
Oct 8, 2011·Toxicon : Official Journal of the International Society on Toxinology·V Quintero-HernándezL D Possani

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