PMID: 7334129Jan 1, 1981Paper

Rapid and efficient immobilization of soluble and small particulate antigens for solid phase radioimmunoassays

Journal of Immunoassay
P H ClevelandD M Worthen

Abstract

A rapid method of antigen immobilization (10 min.) was developed using soluble antigens (bovine serum albumin, epidermal growth factor, and goat IgG) and small particulate antigens (Keyhole limpet hemocynanine and E. coli) by drying them on filter paper discs. This technique results in a high % of the soluble antigen remaining firmly bound, goat IgG (89%), bovine serum albumin (73%). All the antigens we tested retained their antigenicity after drying as detected by [125I] labeled staphylococcal protein A radioimmunoassay. This method of antigen immobilization was compared to adsorption to plastic wells and was found to be much faster (10 min vs 18 hrs) and was 5 times more efficient than adsorption to plastic wells. Using this technique, we were able to detect as little as 40 ng of bovine serum albumin. These characteristics suggest that this technique of soluble antigen immobilization may be useful in rapid detection of antibodies to many different antigens, as well as detecting ng amounts of the antigens directly.

References

Jan 1, 1977·Journal of Immunological Methods·J J LangoneT Borsos
Jan 1, 1978·Journal of Immunological Methods·J J Langone
Jan 1, 1976·Journal of Immunological Methods·J E Herrmann, M F Collins
Jun 30, 1975·Annals of the New York Academy of Sciences·E J RuitenbergJ Buys
Feb 1, 1970·Clinica Chimica Acta; International Journal of Clinical Chemistry·K J CattC Skermer
Dec 22, 1967·Science·K Catt, G W Tregear
Sep 1, 1951·Proceedings of the National Academy of Sciences of the United States of America·D H CampbellL S Lerman

❮ Previous
Next ❯

Citations

Jan 1, 1983·Journal of Immunoassay·K E Hechemy, R L Anacker
Nov 1, 1986·Acta Oto-laryngologica·N K Woolf, J P Harris
Aug 11, 2010·Analytical Biochemistry·Kevin R RupprechtJeffrey R Fishpaugh
May 1, 1985·American Journal of Otolaryngology·J P HarrisA F Ryan
Mar 1, 1985·The Annals of Otology, Rhinology, and Laryngology·J P Harris, A F Ryan
Sep 1, 1986·Clinical Immunology and Immunopathology·A F RyanC W Vogel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.