Rapid and efficient incorporation of tissue factor into liposomes

Journal of Thrombosis and Haemostasis : JTH
S A Smith, J H Morrissey

Abstract

Tissue factor (TF), the physiological trigger of the blood clotting cascade, is also the active ingredient in thromboplastin preparations which are widely used in clotting assays such as the prothrombin time (PT) test. A type I integral membrane protein, TF must be incorporated into suitable phospholipid membranes for full procoagulant activity. Several methods exist for incorporating TF into phospholipid vesicles, typically employing the formation of mixed micelles containing detergent, phospholipid and TF, followed by detergent removal or dilution below the critical micelle concentration (CMC). These methods have certain drawbacks: they may take several days to complete, employ expensive detergents, are difficult to scale up, and do not always result in complete detergent removal. In this study we have investigated the use of a variety of detergents [Triton X-100, octaethylene glycol monododecyl ether (C(12)E(8)), cholate, deoxycholate, and n-octyl-beta-D-glucopyranoside], and the use of adsorbent beads (Bio-Beads SM-2) for removing detergent, in processes to incorporate TF into proteoliposomes with high specific activity in coagulation assays. The method we have developed is rapid and readily scalable, yielding thromboplasti...Continue Reading

References

Nov 13, 1990·European Journal of Biochemistry·P RichardP Gräber
May 1, 1973·Analytical Biochemistry·P W Holloway
Apr 15, 1983·Analytical Biochemistry·J A ReynoldsC Tanford
Jun 9, 1995·Biochimica Et Biophysica Acta·P J KoettersA J Jonas
Jul 17, 1995·Biochimica Et Biophysica Acta·J Lasch
Jun 1, 1994·The Journal of Membrane Biology·J Tranum-JensenJ Vinten
Jan 1, 1993·Methods in Enzymology·J H LawsonK G Mann
Jun 13, 2001·Biochemical and Biophysical Research Communications·G GeQ Lin
Oct 24, 2002·European Journal of Biochemistry·François DevesaYannick Poquet

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Citations

May 13, 2011·The Journal of Biological Chemistry·Narjes TavoosiJames H Morrissey
Apr 21, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Thomas V ColaceScott L Diamond
Oct 23, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Ryan W Muthard, Scott L Diamond
Jun 1, 2014·PloS One·Susanna H M SlukaFelix C Tanner
Jan 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stephanie A SmithJames H Morrissey
Aug 31, 2004·Journal of Thrombosis and Haemostasis : JTH·S A Smith, J H Morrissey
Jul 15, 2006·Journal of Thrombosis and Haemostasis : JTH·S A SmithJ H Morrissey
Apr 5, 2007·Angewandte Chemie·Christian J KastrupRustem F Ismagilov
Apr 26, 2006·Journal of Thrombosis and Haemostasis : JTH·S A SmithJ H Morrissey
Sep 22, 2011·Chemistry : a European Journal·Shiyong Zhang, Yan Zhao
Sep 18, 2012·Journal of Thrombosis and Haemostasis : JTH·J D WelshS L Diamond
Aug 22, 2012·Journal of Thrombosis and Haemostasis : JTH·N J MutchJ H Morrissey
Dec 19, 2013·Journal of Thrombosis and Haemostasis : JTH·A A Onasoga-JarvisK B Neeves
Sep 2, 2015·Biochimica Et Biophysica Acta·Andrej BavdekAndreas Conzelmann
Sep 13, 2014·International Journal of Laboratory Hematology·Kenny M HanssonJohanna Deinum
Sep 14, 2011·Biochemical and Biophysical Research Communications·Jaemin Jeong, Irina M Conboy
Feb 6, 2017·Journal of Burn Care & Research : Official Publication of the American Burn Association·Santiago Rojas CodinaJesús Murat
Jun 8, 2013·Journal of Biomaterials Science. Polymer Edition·Brendan J CaseyPeter Kofinas
Jan 4, 2007·The Journal of Biological Chemistry·Andrew W ShawJames H Morrissey
Nov 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Anders L NielsenHenrik Østergaard
Sep 12, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fabian ItelVolker Endeward
Sep 25, 2019·Journal of Thrombosis and Haemostasis : JTH·Mari EnokssonDonald F Brophy
Sep 29, 2019·Journal of Thrombosis and Haemostasis : JTH·Kathryn G LinkKeith B Neeves
Mar 10, 2007·Blood·Olga PanesDiego Mezzano
Sep 5, 2020·Journal of Thrombosis and Haemostasis : JTH·Fabienne Birkle, James H Morrissey
Sep 21, 2019·The Biochemical Journal·Tina M MisenheimerBradford S Schwartz
Dec 29, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Chengkun LiuFang Huang
Nov 4, 2008·Journal of the American Chemical Society·Sheereen Majd, Michael Mayer
Jun 25, 2010·Journal of the American Chemical Society·Hongkwan Cho, Yan Zhao
Nov 10, 2010·Biomacromolecules·Brendan J CaseyPeter Kofinas
Jul 25, 2013·Biochemistry·Narjes TavoosiJames H Morrissey

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