Rapid and Efficient Purification Method for Small, Hydrophobic, Cationic Bacteriocins: Purification of Lactococcin B and Pediocin PA-1.

Applied and Environmental Microbiology
K VenemaJ Kok

Abstract

The bacteriocins lactococcin B and pediocin PA-1 were purified by ethanol precipitation, preparative isoelectric focusing, and ultrafiltration. The procedure reproducibly leads to high final yields in comparison to the generally low yields obtained by column chromatography. Specifically, during isoelectric focusing no loss of activity occurs. The method, in general, should be applicable to small, hydrophobic, cationic bacteriocins.

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Citations

Apr 1, 2000·Applied and Environmental Microbiology·D GuyonnetJ M Berjeaud
Feb 1, 2011·Expert Opinion on Drug Discovery·Mohammed M Al-Mahrous, Mathew Upton
Mar 29, 2014·Probiotics and Antimicrobial Proteins·Anita Kumari GarsaSuman Kapila
Sep 8, 2017·Critical Reviews in Food Science and Nutrition·Eldin Maliyakkal JohnsonJoo Won Suh
Mar 5, 2004·Journal of Agricultural and Food Chemistry·Chien-Wei WuShann-Tzong Jiang

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