Rapid and high-resolution analysis of geometric polyprenol homologues by connected octadecylsilylated monolithic silica columns in high-performance liquid chromatography

Journal of Separation Science
Takeshi BambaAkio Kobayashi

Abstract

A Chromolith Performance octadecylsilyl (ODS) monolithic silica column (Merck) was compared with a conventional microparticulate ODS-bonded silica column in the high-performance liquid chromatography separation of natural polyprenols. A system comprising two connected monolithic columns afforded an equivalent separation at half the analysis time of the conventional method. Furthermore, ten connected columns achieved a tremendously high-resolution separation, in which the complicated series of homologous polyprenols with geometric isomerism were fully separated.

Citations

Nov 19, 2010·Bioanalysis·Atsuki MatsubaraTakeshi Bamba
Nov 29, 2005·Journal of Bioscience and Bioengineering·Eiichiro Fukusaki, Akio Kobayashi
Dec 20, 2005·Journal of Chromatography. a·Hiroshi KobayashiNobuo Tanaka
Aug 3, 2005·Journal of Lipid Research·Takeshi BambaAkio Kobayashi
Sep 10, 2004·Journal of Separation Science·Karin Cabrera
Nov 27, 2015·Journal of Separation Science·Vyacheslav V Kozlov, Leonid L Danilov
Jan 23, 2009·Journal of Separation Science·Michiel H M van de Meent, Gerhardus J de Jong
Jul 4, 2009·Journal of Separation Science·Hironobu MorisakaMitsuyoshi Ueda
Dec 14, 2004·Journal of Separation Science·Tohru IkegamiNobuo Tanaka

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