Rapid and Highly Sensitive Detection of Variant Creutzfeldt-Jakob Disease Abnormal Prion Protein on Steel Surfaces by Protein Misfolding Cyclic Amplification: Application to Prion Decontamination Studies

PloS One
Maxime BelondradeDaisy Bougard

Abstract

The prevalence of variant Creutzfeldt-Jakob disease (vCJD) in the population remains uncertain, although it has been estimated that 1 in 2000 people in the United Kingdom are positive for abnormal prion protein (PrPTSE) by a recent survey of archived appendix tissues. The prominent lymphotropism of vCJD prions raises the possibility that some surgical procedures may be at risk of iatrogenic vCJD transmission in healthcare facilities. It is therefore vital that decontamination procedures applied to medical devices before their reprocessing are thoroughly validated. A current limitation is the lack of a rapid model permissive to human prions. Here, we developed a prion detection assay based on protein misfolding cyclic amplification (PMCA) technology combined with stainless-steel wire surfaces as carriers of prions (Surf-PMCA). This assay allowed the specific detection of minute quantities (10-8 brain dilution) of either human vCJD or ovine scrapie PrPTSE adsorbed onto a single steel wire, within a two week timeframe. Using Surf-PMCA we evaluated the performance of several reference and commercially available prion-specific decontamination procedures. Surprisingly, we found the efficiency of several marketed reagents to remove hu...Continue Reading

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Citations

Apr 18, 2018·The Journal of Pathology. Clinical Research·Marcelo A BarriaMark W Head
Oct 14, 2020·Biomedical Instrumentation & Technology·Camila Q M BrunaKazuko U Graziano
Nov 17, 2020·Frontiers in Bioengineering and Biotechnology·Hasier ErañaJoaquín Castilla
Dec 19, 2020·Frontiers in Bioengineering and Biotechnology·Mohammed MoudjouAngélique Igel-Egalon

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