PMID: 9556754Jun 1, 1997Paper

Rapid and highly sensitive enzyme immunoassay for quantitative determination of tetrodotoxin

Japanese Journal of Medical Science & Biology
K KawatsuT Shibata

Abstract

A monoclonal antibody against tetrodotoxin (TTX) was obtained from Balb/c mice immunized with TTX-bovine serum albumin (BSA) conjugate. The monoclonal antibody was highly specific for TTX and had no cross-reaction to tetrodonic acid, which is a TTX derivative, or gonyautoxins, although a minor cross-reaction to anhydro-tetrodotoxin was observed. The monoclonal antibody neutralized the lethal activity of TTX. By using the monoclonal antibody, a rapid and highly sensitive competitive enzyme immunoassay (EIA) for quantitative analysis of TTX was developed. By the competitive EIA system, TTX can be determined quantitatively in about 30 min (90 min are required if the time for preparation of the solid-phase antigen was included), and the working range for quantitative analysis of TTX was 2-100 ng/ml. In recovery tests and examinations of TTX samples, results of the mouse bioassay and EIA analyses correlated well (r = 0.987). Moreover, it was demonstrated that low concentrations of TTX, which could not be detected by the mouse bioassay, could be determined quantitatively by the competitive EIA.

References

Jul 11, 1975·Science·Y H KimF A Mosher
Nov 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·W S ShestowskyH M Sikorska
Jan 1, 1989·Toxicon : Official Journal of the International Society on Toxinology·T UsagawaT Yasumoto
Jan 1, 1989·Toxicon : Official Journal of the International Society on Toxinology·S WatabeK Yamauchi
Jan 1, 1987·Toxicon : Official Journal of the International Society on Toxinology·M YotsuT Yasumoto
Jan 1, 1985·Toxicon : Official Journal of the International Society on Toxinology·M Nakamura, T Yasumoto
Jan 1, 1985·Toxicon : Official Journal of the International Society on Toxinology·K ShiomiT Kikuchi
Apr 1, 1973·Toxicon : Official Journal of the International Society on Toxinology·T Noguchi, Y Hashimoto
Nov 1, 1964·Chemical & Pharmaceutical Bulletin·K TsudaK Sakai
Aug 1, 1965·Tetrahedron·T GotoY Hirata
Jan 1, 1994·Biophysical Journal·G M Lipkind, H A Fozzard
Sep 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·K Matsumura
Dec 1, 1996·Environmental Health Perspectives·M DosemeciR B Hayes
Jan 31, 1964·Science·H D BUCHWALDF A FUHRMAN
Nov 1, 1964·Proceedings of the Society for Experimental Biology and Medicine·H M JOHNSONK H LEWIS

❮ Previous
Next ❯

Citations

Dec 18, 2013·Analytical and Bioanalytical Chemistry·Kelvin Sze-Yin Leung, Bonnie Mei-Wah Fong
Jan 14, 2011·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Yonekazu Hamano
Dec 7, 2010·Transactions of the Royal Society of Tropical Medicine and Hygiene·Q T IslamD Mebs
Oct 17, 2001·Toxicon : Official Journal of the International Society on Toxinology·M B TanuT Noguchi
Mar 17, 1999·Toxicon : Official Journal of the International Society on Toxinology·K KawatsuY Hamano
Sep 11, 2002·Toxicon : Official Journal of the International Society on Toxinology·Mark P KreuzerGeorge G Guilbault
Apr 5, 2003·Toxicon : Official Journal of the International Society on Toxinology·Yahia MahmudTamao Noguchi
Jul 4, 2013·Analytical and Bioanalytical Chemistry·Natalia VilariñoLuis M Botana
Jun 21, 2015·Journal of Microbiological Methods·Junko SakataYuko Kumeda
Feb 20, 2010·Toxicon : Official Journal of the International Society on Toxinology·Dietrich MebsMari Yotsu-Yamashita
Aug 18, 2009·Toxicon : Official Journal of the International Society on Toxinology·Koichi IkedaOsamu Arakawa
Nov 11, 2008·Toxicon : Official Journal of the International Society on Toxinology·Koichi IkedaOsamu Arakawa
Nov 13, 2007·Toxicon : Official Journal of the International Society on Toxinology·Laymithuna NgyOsamu Arakawa
Dec 1, 2011·Toxicon : Official Journal of the International Society on Toxinology·Monrat ChulanetraWanpen Chaicumpa
Sep 17, 2011·Toxicon : Official Journal of the International Society on Toxinology·Junjie WangOsamu Arakawa
Apr 7, 2012·Toxicon : Official Journal of the International Society on Toxinology·Dietrich MebsOsamu Arakawa
Dec 14, 2011·Marine Drugs·Kelvin Sze-Yin LeungYeuk-Ki Tsoi
Jun 3, 2004·Annals of Neurology·Kevin D Graber, David A Prince
Mar 14, 2012·Biological Procedures Online·Amber N StokesSusannah S French
May 12, 2010·Analytical and Bioanalytical Chemistry·Natalia VilariñoLuis M Botana
Apr 9, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mònica CampàsJorge Diogène
Mar 7, 2017·Toxicon : Official Journal of the International Society on Toxinology·Ryohei TatsunoOsamu Arakawa
Dec 1, 2019·Toxicon : Official Journal of the International Society on Toxinology·Wei GaoOsamu Arakawa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.