Rapid and label-free detection of egg allergen traces in wines by surface plasmon resonance biosensor

Analytical and Bioanalytical Chemistry
Rosa PilolliLinda Monaci

Abstract

The development of a surface plasmon resonance (SPR)-based biosensor tailored to the fast detection of egg-related fining allergens in wines is herein described. Ovalbumin (OVA) was chosen as the target protein to be monitored due to its highest abundance in the egg white powder, a typical fining agent used by the winery industry to promote wine clarification. A direct assay was designed, basing on the use of polyclonal anti-OVA antibody as bio-specific receptor. With the aim of optimizing the assay conditions, different parameters able to influence the final biosensor response were carefully investigated (i.e., pH, ionic strength, and additional surfactant concentration). After the fine tuning of these parameters, the assay was tested in the direct analysis of OVA in commercial wines artificially contaminated with egg white powder at different concentration levels in order to assess the reliability of the biosensor in detecting traces of OVA in complex matrices. The devised assay allowed to trace, in a short analysis time and with a minimal sample pre-treatment required, the presence of egg allergens at the lowest concentration comprised between 0.03 and 0.2 μg/mL. Finally, the response provided by the developed biosensor was ...Continue Reading

References

Jun 1, 2010·Journal of Proteomics·Angelo CeredaPier Giorgio Righetti
Sep 22, 2010·Analytical Chemistry·Sabina Rebe RazMaria G E G Bremer
Jul 23, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·L MonaciA Visconti
Jan 18, 2013·Journal of Agricultural and Food Chemistry·Tatiana CucuBruno De Meulenaer
May 15, 2013·The Analyst·Alina VasilescuSimona Carmen Litescu
Jul 23, 2013·Biosensors & Bioelectronics·Palaniappan SubramanianSabine Szunerits
Aug 14, 2013·Rapid Communications in Mass Spectrometry : RCM·Linda MonaciAngelo Visconti
Dec 6, 2014·Journal of Mass Spectrometry : JMS·Rosa PilolliLinda Monaci

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Citations

Mar 5, 2016·Annual Review of Food Science and Technology·Shridhar K SatheValerie D Zaffran
Jun 1, 2016·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·Martin Röder, Wolfgang Weber
Jan 4, 2017·Sensors·Yung-Shin SunJin-Cherng Hsu
Apr 21, 2018·Journal of the Science of Food and Agriculture·Anita SpychajMagdalena Montowska
Dec 9, 2016·Analytical and Bioanalytical Chemistry·Rosa PilolliLinda Monaci
Nov 25, 2020·Biosensors·Syed Akif Raza KazmiJean-Francois Masson
Jan 26, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shin-Woong KimTakanori Uzawa
Jun 2, 2021·Critical Reviews in Food Science and Nutrition·Sara BenedéElena Molina
Jun 19, 2021·International Journal of Nanomedicine·Taiki MiyazawaTeruo Miyazawa

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