Rapid and reproducible Agrobacterium-mediated transformation of sorghum

Plant Cell Reports
Arlene HoweTom Clemente

Abstract

A rapid and reproducible Agrobacterium-mediated transformation protocol for sorghum has been developed. The protocol uses the nptII selectable marker gene with either of the aminoglycosides geneticin or paromomycin. A screen of various A. tumefaciens strains revealed that a novel C58 nopaline chromosomal background carrying the chrysanthopine disarmed Ti plasmid pTiKPSF(2), designated NTL(4)/Chry5, was most efficient for gene transfer to sorghum immature embryos. A NTL(4)/Chry5 transconjugant harboring the pPTN290 binary plasmid, which carries nptII and GUSPlus expression cassettes, was used in a series of stable transformation experiments with Tx430 and C2-97 sorghum genotypes and approximately 80% of these transformation experiments resulted in the recovery of at least one transgenic event. The transformation frequencies among the successful experiments ranged from 0.3 to 4.5%, with the average transformation frequency being approximately 1% for both genotypes. Over 97% of the transgenic events were successfully established in the greenhouse and were fully fertile. Co-expression of GUSPlus occurred in 89% of the transgenic T(0) events. Seed set for the primary transgenic plants ranged from 145 to 1400 seed/plant. Analysis of ...Continue Reading

References

Apr 1, 1990·Journal of Virology·J C Carrington, D D Freed
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M D ChiltonE W Nester
Sep 1, 1994·Plant Molecular Biology·P HajdukiewiczP Maliga
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A M CasasP M Hasegawa
Feb 24, 2001·Molecular Plant-microbe Interactions : MPMI·Z Q LuoS K Farrand
Feb 24, 2001·Plant Molecular Biology·Z Y ZhaoD Pierce
Jan 22, 2005·PLoS Biology·Joseph A BedellRobert A Martienssen
Apr 20, 2005·Genome Génome / Conseil National De Recherches Canada·Zhensheng GaoG H Liang
Sep 1, 1991·Applied and Environmental Microbiology·A L Bush, S G Pueppke

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Citations

Aug 25, 2009·Plant Cell Reports·N Shiva PrakashRaghava S Boddupalli
Jun 11, 2010·Plant Cell Reports·Anshu Raghuwanshi, Robert G Birch
Jan 22, 2008·Journal of Industrial Microbiology & Biotechnology·Gautam SarathKenneth P Vogel
Feb 15, 2011·Plant Molecular Biology·Tejinder Kumar MallTom Clemente
Oct 1, 2009·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·V Girijashankar, V Swathisree
Jun 29, 2013·Genome Biology·Savio de Siqueira FerreiraGlaucia Mendes Souza
Dec 30, 2015·The Plant Journal : for Cell and Molecular Biology·Erin D ScullyScott E Sattler
Feb 23, 2012·Plant Biotechnology Journal·Tejinder KumarTom Clemente
Jan 7, 2010·Plant Biotechnology Journal·Amir SattarzadehDavid B Stern
Jun 24, 2016·Molecular Biology and Evolution·Hugo E CuevasAndrew H Paterson
Oct 3, 2018·International Journal of Molecular Sciences·Rana Imtiaz AhmedYingzhen Kong
Jul 7, 2018·Plant Biotechnology Journal·Chase KempinskiJoe Chappell
Oct 29, 2018·Current Protocols in Plant Biology·Kimberly Nelson-VasilchikAlbert Kausch
Sep 28, 2018·The Plant Journal : for Cell and Molecular Biology·Richard E BoylesStephen Kresovich
Apr 21, 2017·Frontiers in Plant Science·Pamela A PeñaTom E Clemente
Jan 1, 2014·In Vitro Cellular & Developmental Biology. Plant : Journal of the Tissue Culture Association·Emily WuZuo-Yu Zhao
Aug 28, 2020·Preparative Biochemistry & Biotechnology·Samaneh Rahamooz-HaghighiHossein Danafar
Nov 6, 2021·Planta·Zhanguo XinGeoffrey Morris

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