Rapid and sensitive detection of Staphylococcus species in milk by ELISA based on monodisperse magnetic particles

Veterinary Microbiology
S P YazdankhahE Olsen

Abstract

Staphylococcus spp. are the single most important cause of mastitis in dairy cows. Aiming for a rapid and sensitive method for detection of staphylococci, a magnetic bead-based enzyme-linked immunosorbent assay (ELISA) employing MAb for the detection of staphylococci in milk was developed. Pretreatment of the sample with lysostaphin increased the sensitivity of the assay. The method is specific for most of staphylococci which cause mastitis in bovines, detects between 10(4)-10(5) organisms per ml and takes three hours. Thus, the principle for staphylococci ELISA based on monodisperse magnetic beads is a way for rapid detection of staphylococci in milk and possibly also for detecting staphylococci by direct testing of other clinical specimens. The system may be automated.

References

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Citations

Mar 17, 2004·Journal of Pharmaceutical and Biomedical Analysis·F N LamariN K Karamanos
Oct 3, 2003·Biomagnetic Research and Technology·ZM SaiyedCN Ramchand
Feb 11, 2014·Pakistan Journal of Biological Sciences : PJBS·Rajib DebSushil Kumar
Jun 21, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M H Fabres-KleinA O B Ribon
Jul 21, 2009·Trends in Biotechnology·Caroline ViguierRichard O'Kennedy
Oct 10, 2015·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Carla M DuarteRicardo Bexiga
Jun 14, 2017·Journal of Applied Microbiology·V Templier, Y Roupioz
Jul 3, 1999·Nutrition Reviews·R D Semba, M C Neville

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