Rapid and sensitive diagnosis of Toxoplasma gondii infections by PCR

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
S JalalESCMID Study Group on Toxoplasmosis

Abstract

DNA was extracted with a modified Qiagen DNA Mini Kit method from 20 clinical samples and was amplified by PCR using specific primers for the T. gondii B1 gene. T. gondii was detected correctly in 18 of the 20 clinical samples in < 5 h, with a detection limit of two parasites/sample. The results were in good agreement with those obtained by a more complicated and time-consuming procedure involving two-step nested PCR and either liquid hybridisation or colorimetric detection using internal probes.

References

Jan 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·U GrossJ Heesemann
Jan 1, 1993·Journal of Medical Microbiology·A W JossD O Ho-Yen
Oct 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E C GuyP Ambroise-Thomas
Aug 12, 1999·Ophthalmology·J G MontoyaJ S Remington
Aug 23, 2001·Scandinavian Journal of Infectious Diseases·I JulanderB Evengård

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Citations

Mar 28, 2012·Vector Borne and Zoonotic Diseases·Jiping LiJun Qian
Apr 14, 2009·Applied and Environmental Microbiology·Wenli YangLihua Xiao
Sep 28, 2012·Clinical and Vaccine Immunology : CVI·Andrea-Romana PrusaDavid C Kasper
Sep 22, 2012·Interdisciplinary Perspectives on Infectious Diseases·Virgen J Castillo-MoralesAntonio Ortega-Pacheco
Dec 10, 2009·Future Microbiology·Vera Lucia Pereira-ChioccolaChunlei Su
Jul 11, 2013·BioMed Research International·A Ortega-PachecoM Jiménez-Coello
Oct 30, 2004·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Benjamin EdvinssonUNKNOWN ECSMID Study Group on Toxoplasmosis
May 3, 2008·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Benjamin EdvinssonBirgitta Evengård
Mar 17, 2010·Journal of Veterinary Emergency and Critical Care·Peter P Nghiem, Scott J Schatzberg
Nov 23, 2005·International Journal for Parasitology·Damer P BlakeAdrian L Smith
Oct 22, 2010·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Rafael Tonini MesquitaVera Lucia Pereira-Chioccola
May 25, 2015·Asian Pacific Journal of Tropical Medicine·Shirzad FallahiAli Haghighi
Jan 31, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·B EdvinssonUNKNOWN ESCMID Study Group for Toxoplasmosis
Sep 24, 2016·Foodborne Pathogens and Disease·Ivonne Beatriz Hernández-CortazarMatilde Jimenez-Coello
Jan 26, 2018·Journal of Wildlife Diseases·Radka LukášováM Justin O'Riain
Jan 8, 2019·Vector Borne and Zoonotic Diseases·Antonio Gomez-RiosMatilde Jiménez-Coello
Jan 12, 2021·Journal Für Verbraucherschutz Und Lebensmittelsicherheit = Journal of Consumer Protection and Food Safety·Amin YousefvandShirin Mohammadyari
Jun 12, 2021·Expert Review of Anti-infective Therapy·Marie Gladys RobertHervé Pelloux

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Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
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