Rapid and sensitive LC-MS/MS method for the analysis of antibiotic linezolid on dried blood spot

Journal of Pharmaceutical and Biomedical Analysis
Giancarlo la MarcaLuisa Galli

Abstract

Linezolid is a new drug from the oxazolidinone class of antibiotics used against mycobacteria and multi-drug resistant (MDR) Gram-positive bacterial infections, which may are also glycopeptide-resistant. The drug usage in pediatric age needs an accurate drug monitoring for effective patient management. The aim of this study was to evaluate the use of dried blood spot (DBS) specimens to determinate linezolid levels during treatment. Advantages of DBS include short collection time, low invasiveness, ease and low cost of sample collection, transport and storage. The analysis was performed in LC-MS/MS operating in positive ion mode and multiple reaction monitoring (MRM) mode. The calibration curve in matrix was linear in the concentration range of 1-100 mg/L with correlation coefficient value of 0.9987. Intraday and interday coefficients of variation were within 3.6% and 13.0%, respectively. We also tested the thermal and temporal drug stability in dried blood spots at four different temperatures to evaluate the risks of sample delivery in different conditions. The short term stability studies showed that linezolid concentration remained stable for at least one month under all the conditions tested. This new assay has favorable cha...Continue Reading

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Citations

Aug 27, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lorena BaiettoFrancesco Giuseppe De Rosa
Sep 11, 2014·Clinical Pharmacokinetics·Abraham J WilhelmEleonora L Swart
Jan 17, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hua-chen YuYi-mu Lin
Apr 3, 2014·Drug Testing and Analysis·Abhisheak SharmaJawahar Lal
Feb 14, 2015·Biomedical Chromatography : BMC·Shuuji HaraYasushi Takamatsu
May 11, 2016·Clinical Biochemistry·Maria Shipkova, Dobrin Svinarov
May 18, 2016·Clinical Biochemistry·Marina Venzon AntunesRafael Linden
Apr 19, 2016·Expert Opinion on Drug Metabolism & Toxicology·Suzanne L ParkerJason A Roberts
Jul 9, 2016·Journal of Pharmaceutical and Biomedical Analysis·Yeliz EnderleJürgen Burhenne
Oct 11, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Samah A MohammedHytham M Ahmed
Apr 25, 2019·Critical Reviews in Analytical Chemistry·Guilherme Felipe Dos Santos FernandesJean Leandro Dos Santos
Sep 25, 2014·Mass Spectrometry Reviews·Michel WagnerGérard Hopfgartner
Oct 28, 2019·Therapeutic Drug Monitoring·Gauri G RaoUNKNOWN IATDMCT Antimicrobial Scientific Committee
Apr 9, 2019·Critical Reviews in Analytical Chemistry·K S KokilambigaiJ Kavitha
Nov 14, 2019·Biosensors·Vivian GarzónSandra Bustamante

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