Rapid and sensitive method for detection of hepatitis C virus RNA by using silica particles.

Journal of Clinical Microbiology
R C CheungH B Greenberg

Abstract

We describe a rapid, sensitive, and economic method for detection of hepatitis C virus (HCV) RNA. This method uses silica particles for purification of nucleic acid and then a modified reverse transcription-PCR that minimizes the risk of contamination and reduces the amount of reagents used. We found purification by silica particles to be at least as sensitive and in certain circumstances more sensitive than that by traditional phenol-chloroform extraction. This improved sensitivity may be due to more efficient recovery of HCV RNA by silica particles. HCV RNA appears to bind to silica particles in a saturable fashion, and the addition of extraneous nucleic acids (salmon sperm DNA or tRNA) decreases the binding in a dose-related fashion. The reverse transcription-PCR is performed by using a modified single tube method which further simplifies and reduces the cost of this assay. Finally, this method may be applied to clinical specimens such as liver tissue.

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Citations

Nov 26, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L FrangeulJ M Huraux
Jan 21, 2000·Acta Veterinaria Hungarica·I KissS Belák
Oct 5, 2006·Journal of Forensic Sciences·Ram KishoreMartin R Buoncristiani
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Aug 16, 2016·Acta Medica (Hradec Králové)·Martin BeránekVladimír Palička
Mar 10, 2000·The American Journal of Gastroenterology·R C Cheung
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Mar 11, 2017·Virology Journal·Nerea FontechaMiren Basaras
May 23, 1998·Journal of Clinical Microbiology·J E FoleyN C Pedersen
Sep 1, 1995·Journal of Clinical Microbiology·G MorsicaA Lazzarin

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