Rapid and Sensitive Quantification of Osimertinib in Human Plasma Using a Fully Validated MALDI-IM-MS/MS Assay

Cancers
Margaux FresnaisRémi Longuespée

Abstract

The third-generation tyrosine kinase inhibitor (TKI), osimertinib, has revolutionized the treatment of patients with non-small cell lung carcinoma with epidermal growth factor receptor (EGFR)-activating mutation, and resistant to first- and second-generation TKIs. Osimertinib is now also proposed as a first-line therapy, thus extending the scope of applications in lung oncology. Personalized medicine approaches are still necessary to monitor if patients are exposed to adequate concentrations of osimertinib during their treatment. It would also help to understand the appearance of new resistances in patients after several months of dosing with osimertinib. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is currently the gold standard for the quantification of drugs in plasma enabling pharmacokinetic analyses and patient monitoring. In the present study, we propose an alternative to LC-MS/MS methods for the rapid and sensitive quantification of osimertinib in plasma using matrix-assisted laser desorption/ionization (MALDI) -MS. The presented assay requires only 3 min per sample for their preparation, analysis, and data extraction, and less than 3 h for quantification. A lower limit of quantification (LLOQ) of 5 ng/mL in...Continue Reading

References

Jan 30, 2004·Analytical and Bioanalytical Chemistry·DebraLynn Mims, David Hercules
Dec 30, 2014·Journal of Pharmaceutical and Biomedical Analysis·Ania Cabrales-RicoLuis Javier González
Dec 24, 2016·British Journal of Clinical Pharmacology·Kathryn BrownEugène Cox
Nov 21, 2017·The New England Journal of Medicine·Jean-Charles SoriaUNKNOWN FLAURA Investigators
Mar 4, 2018·Acta Neuropathologica Communications·Rémi LonguespéeStefan Pusch
Apr 11, 2018·Journal of the American Society for Mass Spectrometry·Yulin QiDietrich A Volmer
Apr 21, 2018·Archives of Disease in Childhood·Charlotte I S BarkerMartin Offringa
Sep 4, 2018·Journal of Thoracic Disease·Tao JiangUNKNOWN written on behalf of the AME Lung Cancer Collaborative Group
Feb 2, 2019·Mass Spectrometry Reviews·Valérie GabelicaThomas Wyttenbach
Mar 20, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·G D Marijn VeermanPeter de Bruijn
Jun 21, 2019·Bioanalysis·Richard MitchellPhillip Turpin
Oct 29, 2019·Rapid Communications in Mass Spectrometry : RCM·Margaux FresnaisRémi Longuespée
Jan 24, 2020·Journal of the American Society for Mass Spectrometry·Margaux FresnaisRémi Longuespée
Oct 1, 2018·Annals of Oncology : Official Journal of the European Society for Medical Oncology·D PlanchardUNKNOWN ESMO Guidelines Committee. Electronic address: clinicalguidelines@esmo.org

❮ Previous
Next ❯

Citations

Mar 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Margaux FresnaisRémi Longuespée
Sep 4, 2020·British Journal of Clinical Pharmacology·Rémi LonguespéeWalter E Haefeli

❮ Previous
Next ❯

Software Mentioned

Prism
MobA
ApexTrack
MassI
GraphPad
MassLynx
FLAURA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.