Rapid and sensitive spectrofluorimetric determination of enrofloxacin, levofloxacin and ofloxacin with 2,3,5,6-tetrachloro-p-benzoquinone

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Sevgi Tatar Ulu

Abstract

A highly sensitive spectrofluorimetric method was developed for the first time, for the analysis of three fluoroquinolones (FQ) antibacterials, namely enrofloxacin (ENR), levofloxacin (LEV) and ofloxacin (OFL) in pharmaceutical preparations through charge transfer (CT) complex formation with 2,3,5,6-tetrachloro-p-benzoquinone (chloranil,CLA). At the optimum reaction conditions, the FQ-CLA complexes showed excitation maxima ranging from 359 to 363nm and emission maxima ranging from 442 to 488nm. Rectilinear calibration graphs were obtained in the concentration range of 50-1000, 50-1000 and 25-500ngmL(-1) for ENR, LEV and OFL, respectively. The detection limit was found to be 17ngmL(-1) for ENR, 17ngmL(-1) for LEV, 8ngmL(-1) for OFL, respectively. Excipients used as additive in commercial formulations did not interfere in the analysis. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The proposed method was successfully applied to the analysis of pharmaceutical preparations. The results obtained were in good agreement with those obtained using the official method; no significant difference in the accuracy and precision as revealed by the accepted ...Continue Reading

References

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Citations

Jun 9, 2014·Chemosphere·Yolanda PatiñoSalvador Ordóñez
Jan 24, 2012·Journal of Hazardous Materials·Hongbo PengBaoshan Xing
Mar 20, 2010·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hao WuLi-Ming Du
Aug 28, 2012·Environmental Pollution·Bo PanBaoshan Xing
Apr 16, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Zhao TongZhang Haixia
Jul 21, 2012·Journal of Chromatographic Science·Zhili FangWeiguang Zhang

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