Rapid and simple detection of Tamiflu-resistant influenza virus: Development of oseltamivir derivative-based lateral flow biosensor for point-of-care (POC) diagnostics

Scientific Reports
Seul Gee HwangEun-Kyung Lim

Abstract

We have developed a novel oseltamivir derivative (oseltamivir hexylthiol; OHT) that exhibits a higher binding affinity for Tamiflu-resistant virus (Tamiflu resistance) than for the wild-type virus (Tamiflu-susceptible virus; WT) as an antibody. First, OHT-modified gold nanoparticles (OHT-GNPs) are used in a simple colorimetric assay as nanoprobes for the Tamiflu-resistant virus. In the presence of Tamiflu-resistant virus, they show a colorimetric change from deep red to purple because of the OHT-GNP aggregation driven by strong interactions between OHT and neuraminidase (NA) on the surface of the Tamiflu-resistance. Moreover, the color gradually turns purple as the concentration of the Tamiflu-resistant virus increases, allowing the determination of the presence of the virus with the naked eye. Furthermore, an OHT-based lateral flow assay (LFA) has been developed as a rapid and easy detection device for Tamiflu resistance. It shows detection specificity for various virus concentrations of Tamiflu-resistant virus even for the mixture of WT and Tamiflu-resistant viruses, where the limit of detection (LOD) is 5 × 102 ~ 103 PFU per test (=1 × 104 PFU/mL). It has been confirmed that this platform can provide accurate information on ...Continue Reading

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Citations

Dec 14, 2018·Micromachines·Taek LeeMin-Ho Lee
Jul 24, 2020·International Journal of Molecular Sciences·Navid RabieeRajender S Varma
Oct 30, 2020·Chembiochem : a European Journal of Chemical Biology·Hemanth MaddaliDeirdre M O'Carroll
Feb 16, 2021·Advanced Materials·Eunjung KimSeungjoo Haam
Jan 20, 2021·Analytica Chimica Acta·Micaela L EverittIan M White
Feb 20, 2021·Materials Science for Energy Technologies·Victoria Xin Ting ZhaoXiaodong Zhou
Apr 17, 2021·Analytical Methods : Advancing Methods and Applications·Natpapas WiriyachaipornKanyawan Ponlamuangdee
Jan 12, 2019·Analytical Chemistry·Wen RenJoseph Irudayaraj

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Methods Mentioned

BETA
PCR
column chromatography
nuclear magnetic resonance
X-ray

Software Mentioned

Prism
BioEdit
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