Rapid and simple determination of oxolinic acid and oxytetracycline in the shell of the blue mussel (Mytilus edulis) by high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Sciences and Applications
H PouliquenL Pinault

Abstract

A simple procedure for the determination of oxolinic acid (OA) and oxytetracycline (OTC), two antibacterial agents, in the shell of the blue mussel (Mytilus edulis), using reversed-phase high-performance liquid chromatography is described. Liquid chromatography was performed on a 5-microm LiChroSpher 100 RP-18E column using acetonitrile and a 0.02 M orthophosphoric acid solution as the mobile phase, with ultraviolet detection. After roughly grinding the shell, drugs were extracted using a methanolic oxalic acid solution. Linearity and precision were checked over the concentration range 0.04-0.32 microg/g. Limits of detection of OA and OTC were 0.012 and 0.008 microg/g, respectively. Mean extraction recoveries of OA and OTC from mussel shell were 72.9 and 65.4%, respectively. To demonstrate the usefulness of the analytical procedure, an experimental study was performed in blue mussels exposed to the drugs for eight days.

Citations

Oct 31, 2008·Talanta·F BelalA M Al-Obaid
Mar 10, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·M TourakiC Prokopiou
Aug 21, 2012·PloS One·Alejandro H BuschmannFelipe C Cabello
Aug 31, 2010·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Shoujun YuanNyles G Peterson
Jan 8, 1999·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·I Poher, G Blanc
Feb 28, 2002·Journal of Chromatography. a·J A Hernández-ArteserosM D Prat
Jul 15, 2000·Journal of Chromatography. a·H OkaH Matsumoto

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