Rapid and simple method for in vivo ex utero development of mouse embryo explants

Differentiation; Research in Biological Diversity
André B GonçalvesMarianne Deries

Abstract

The in utero development of mammals drastically reduces the accessibility of the mammalian embryo and therefore limits the range of experimental manipulation that can be done to study functions of genes or signaling pathways during embryo development. Over the past decades, tissue and organ-like culture methods have been developed with the intention of reproducing in vivo situations. Developing accessible and simple techniques to study and manipulate embryos is an everlasting challenge. Herein, we describe a reliable and quick technique to culture mid-gestation explanted mouse embryos on top of a floating membrane filter in a defined medium. Viability of the cultured tissues was assessed by apoptosis and proliferation analysis showing that cell proliferation is normal and there is only a slight increase in apoptosis after 12h of culture compared to embryos developing in utero. Moreover, differentiation and morphogenesis proceed normally as assessed by 3D imaging of the transformation of the myotome into deep back muscles. Not only does muscle cell differentiation occur as expected, but so do extracellular matrix organization and the characteristic splitting of the myotome into the three epaxial muscle groups. Our culture method...Continue Reading

References

May 1, 1986·The Journal of Cell Biology·K L CrossinG M Edelman
Mar 1, 1980·Analytical Biochemistry·D Barnes, G Sato
May 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J A Bolker
Mar 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J B MillerJ A Dominov
Feb 12, 1998·Mechanisms of Development·P P Tam, R R Behringer
Apr 2, 1999·Science·M F PittengerD R Marshak
Sep 27, 2000·Human Molecular Genetics·V Allamand, K P Campbell
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·A KarpasB H Czepulkowski
May 11, 2002·Nature Reviews. Cancer·John R Masters
Dec 6, 2002·Nature·Allan Bradley
Mar 2, 2006·Developmental Neuroscience·Petur H PetersenWeimin Zhong
Mar 24, 2006·Development·Fernanda BajancaSólveig Thorsteinsdóttir
Sep 27, 2006·Journal of Cellular Physiology·Marcia V Fournier, Katherine J Martin
Feb 14, 2007·Circulation·Katherine E Yutzey, Jeffrey Robbins
Mar 7, 2007·Nature Reviews. Genetics·Ronald A Jenner, Matthew A Wills
Apr 10, 2008·Cell Stem Cell·Paolo BiancoPaul J Simmons
May 29, 2008·Development, Growth & Differentiation·Masanori TakahashiNoriko Osumi
Dec 24, 2008·Genes & Nutrition·Giovanna MazzoleniN Steimberg
Oct 9, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Claire Fournier-ThibaultJean-Loup Duband
Mar 1, 1911·The Journal of Experimental Medicine·A Carrel, M T Burrows
May 1, 1912·The Journal of Experimental Medicine·A Carrel
Jan 27, 2010·The Mount Sinai Journal of Medicine, New York·Gregory A ElderRita De Gasperi
Sep 25, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Marianne DeriesMarilyn J Duxson
Dec 1, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Marianne DeriesSólveig Thorsteinsdóttir
Sep 6, 2012·Molecular and Cellular Biology·Hadi KhalilChristian Widmann
Nov 10, 2013·Birth Defects Research. Part C, Embryo Today : Reviews·Sky Feuer, Paolo Rinaudo
Nov 19, 2013·Cell·Daniel Muñoz-EspínManuel Serrano
May 17, 2014·Assay and Drug Development Technologies·Rasheena EdmondsonLiju Yang

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis