Rapid and simple quantitative measurement of alpha-fetoprotein by combining immunochromatographic strip test and artificial neural network image analysis system

Clinica Chimica Acta; International Journal of Clinical Chemistry
Louise ChuangShiang-Bin Jong

Abstract

Quantitative immunochromatographic strip (ICS) assay can facilitate clinical diagnosis by providing more information than traditional qualitative or semiquantitative strip assay. We constructed a human serum alpha-fetoprotein (AFP) measurement system by combining semiquantitative ICS tests and artificial neural network (ANN) image analysis system [immunochromatographic strip analyzed by artificial neural network image analysis system (IAIS)]. After ICS tests completed, the AFP concentration can be obtained from analysis of IAIS software. The equipment required are commercial semiquantitative AFP dipstick, optical scanner, centrifuge (option), personal computer, and software of IAIS. The serum AFP concentrations measured by IAIS were strongly correlated (r = 0.9971) with that by RIA. Using Bland-Altman analysis, the IAIS achieved clinical acceptable limits of agreement in comparison with RIA. The within-run precision of IAIS, expressed as coefficients of variation (CV), at 81.7 ng/ml was 1.50% and, at 244.4 ng/ml, was 1.09%. The measurement of serum AFP by IAIS can be completed in 20 min. The newly constructed quantitative immunochromatographic strip assay (IAIS) is a simple, rapid, and reliable method for serum AFP measurement....Continue Reading

References

Sep 1, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·A van AmerongenJ H Wichers
Sep 10, 2002·Ultrasound in Medicine & Biology·Louise ChuangFong-Ming Chang
Jan 1, 1956·Scandinavian Journal of Clinical and Laboratory Investigation·C G BERGSTRAND, B CZAR
Dec 23, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sunga ChoiSang Wook Oh

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Citations

Jul 25, 2015·Scandinavian Journal of Clinical and Laboratory Investigation·Fan ZhouTong Wang
Jan 2, 2007·Analytical Biochemistry·Shwu-Jinng WangYung-Chuan Liu
Apr 29, 2014·IEEE Transactions on Medical Imaging·Nianyin ZengTerry Young
Jan 20, 2011·IEEE Transactions on Bio-medical Engineering·Nianyin ZengXiaohui Liu

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