Rapid and Simple Species Identification of Cicada Exuviae Using COI-Based SCAR Assay

Insects
Pureum NohByeong Cheol Moon

Abstract

Cicadidae periostracum (CP), the medicinal name of cicada exuviae, is well-known insect-derived traditional medicine with various pharmacological effects, e.g., anticonvulsive, anti-inflammatory, antitussive, and anticancer effects; it is also beneficial for the treatment of Parkinson's disease. For appropriate CP application, accurate species identification is essential. The Korean pharmacopoeia and the pharmacopoeia of the People's Republic of China define Cryptotympana atrata as the only authentic source of CP. Species identification of commercially distributed CP based on morphological features, however, is difficult because of the combined packaging of many cicada exuviae in markets, damage during distribution, and processing into powder form. DNA-based molecular markers are an excellent alternative to morphological detection. In this study, the mitochondrial cytochrome c oxidase subunit I sequences of C. atrata, Meimuna opalifera, Platypleura kaempferi, and Hyalessa maculaticollis were analyzed. On the basis of sequence alignments, we developed sequence-characterized amplified-region (SCAR) markers for efficient species identification. These markers successfully discriminated C. atrata from the three other cicada species,...Continue Reading

References

Jul 15, 1999·Journal of Ethnopharmacology·R W Pemberton
Sep 3, 1999·Journal of Ethnopharmacology·T Y ShinH M Kim
Mar 5, 2003·Proceedings. Biological Sciences·Paul D N HebertJeremy R deWaard
Aug 22, 2006·Bioorganic & Medicinal Chemistry·Ming-Zhe XuHo-Yong Park
Oct 25, 2008·Health Policy·Judy Xu, Yue Yang
Jan 1, 2010·Molecular Ecology Resources·F-W LiC-N Wang
Dec 8, 2011·Molecular Ecology Resources·Jing CheYa-Ping Zhang
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Aug 5, 2014·Biotechnology Advances·Shilin ChenChristine Leon
May 3, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Haidan YuanGuangchun Piao
Jun 10, 2016·Journal of Pharmacopuncture·Bong-Keun SongSungchul Kim
Mar 29, 2018·BMC Complementary and Alternative Medicine·Maslin OsathanunkulPanagiotis Madesis
Aug 29, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pureum NohByeong Cheol Moon
Nov 28, 2019·Oxidative Medicine and Cellular Longevity·Hye-Sun LimGunhyuk Park

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Methods Mentioned

BETA
PCR
electrophoresis
PCRs

Software Mentioned

BLAST
MEGA
BioEdit
ClustalW

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