Rapid and simple UPLC-MS/MS method for precise phytochelatin quantification in alga extracts

Analytical and Bioanalytical Chemistry
Anja BräutigamDirk Schaumlöffel

Abstract

Quantitative phytochelatin (PC) analysis is, due to oxidation sensitivity of the PCs, matrix effects, and time consuming sample preparation, still a challenging analytical task. In this study, a rapid, simple, and sensitive method for precise determination of native PCs in crude extracts of the green alga Chlamydomonas reinhardtii was developed. Algae were exposed 48 h to 70 μM Cd. Coupling of ultra performance liquid chromatography and electrospray ionization tandem mass spectrometry with multi-reaction mode transitions for detection permitted the required short-time, high-resolution separation and detection specificity. Thus, under optimized chromatographic conditions, 10 thiol peptides were baseline-separated within 7 min. Relative detection limits in the nanomolar range in microliter sample volumes were achieved (corresponding to absolute detection limits at femtomole level). Next to glutathione (GSH), the most abundant cadmium-induced PCs in C. reinhardtii, namely CysGSH, PC(2), PC(3), CysPC(2), and CysPC(3), were quantified with high reproducibility at concentrations between 15 and 198 nmol g(-1) fresh weight. The biological variation of PC synthesis of nine independently grown alga cultures was determined to be on averag...Continue Reading

References

Sep 23, 1994·Journal of Chromatography. B, Biomedical Applications·K Shimada, K Mitamura
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Jul 8, 2005·Analytical and Bioanalytical Chemistry·M H A El-ZohriH Frank
Dec 31, 2005·Journal of Plant Physiology·Stephan Clemens
Jan 1, 1992·Plant Physiology·G Howe, S Merchant
May 16, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yanbing Zu
Jul 11, 2009·Analytical and Bioanalytical Chemistry·Anja BräutigamDirk Wesenberg
Jul 22, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yusuke IwasakiHiroyuki Nakazawa
Nov 13, 2010·Metallomics : Integrated Biometal Science·Anja BräutigamDirk Wesenberg

❮ Previous
Next ❯

Citations

Apr 2, 2011·Analytical and Bioanalytical Chemistry·Dirk Schaumlöffel, Andreas Tholey
Feb 6, 2013·PLoS Neglected Tropical Diseases·Coraline RigouinDavid L Williams
Jul 25, 2013·Environmental Science and Pollution Research International·R FernándezA González
Oct 15, 2013·Biotechnology Journal·Tsung-Hua LeeHsiang-Yu Wang
Apr 20, 2012·Analytical and Bioanalytical Chemistry·B Alan Wood, Jörg Feldmann
Aug 9, 2011·Plant, Cell & Environment·Anja BräutigamDirk Wesenberg
Mar 7, 2021·Metabolites·Andrew Davic, Michael Cascio
Mar 20, 2021·Inorganic Chemistry·Joanna WątłyArtur Krężel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.