PMID: 11334346May 4, 2001Paper

Rapid and simultaneous determination of mycophenolic acid and its glucuronide conjugate in human plasma by ion-pair reversed-phase high-performance liquid chromatography using isocratic elution

Journal of Chromatography. B, Biomedical Sciences and Applications
H HosotsuboH Sugimoto

Abstract

A high-performance liquid chromatographic method has been developed for the simultaneous determination of mycophenolic acid (MPA) and its glucuronide conjugate (MPAG) in human plasma. The method involves protein precipitation with acetonitrile, followed by ion-pair reversed-phase chromatography on C18 column, with a 40 mM tetrabutyl ammonium bromide (TBA)-acetonitrile (65:35, v/v) mobile phase. A 20-microl volume of clear supernatant was injected after centrifugation, and the eluent was monitored at 304 nm. No interference was found either with endogenous substances or with many concurrently used drugs, indicating a good selectivity for the procedure. Calibration curves were linear over a concentration range of 0.5-20.0 microg/ml for MPA and 5-200 microg/ml for MPAG. The accuracy of the method is good, that is, the relative error is below 5%. The intra- and inter-day reproducibility of the analytical method is adequate with relative statistical deviations of 6% or below. The limits of quantification for MPA and MPAG were lower than 0.5 and 5.0 microg/ml, respectively, using 50 microl of plasma. The method was used to determine the pharmacokinetic parameters of MPA and MPAG following oral administration in a patient with renal t...Continue Reading

References

Jan 1, 1990·Journal of Pharmaceutical and Biomedical Analysis·G P Carr, J C Wahlich
Nov 21, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·J J HuangT L Tarnowski

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Citations

Apr 29, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wai-Ping YauEli Chan
Sep 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Antonella ArestaGiuseppe Grandaliano
Oct 18, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·D TeshimaR Oishi
Jun 15, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·An-Sofie C DecaveleAlain G Verstraete
Oct 13, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xavier DelavenneThierry Basset
Nov 26, 2008·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Benoit BlanchetMichel Tod
Feb 28, 2003·Journal of Clinical Pharmacy and Therapeutics·D TeshimaR Oishi
Feb 20, 2007·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·A I MohrK Meyer
Apr 11, 2014·Therapeutic Drug Monitoring·Michael O AlbertiKathleen A Kelly

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