Rapid and specific detection of escherichia coli serogroups O26, O45, O103, O111, O121, O145, and O157 in ground beef, beef trim, and produce by loop-mediated isothermal amplification.

Applied and Environmental Microbiology
Fei WangBeilei Ge

Abstract

Escherichia coli O157 and six additional serogroups of Shiga toxin-producing E. coli (STEC) (O26, O45, O103, O111, O121, and O145) account for the majority of STEC infections in the United States. In this study, O serogroup-specific genes (wzx or wzy) were used to design loop-mediated isothermal amplification (LAMP) assays for the rapid and specific detection of these leading STEC serogroups. The assays were evaluated in pure culture and spiked food samples (ground beef, beef trim, lettuce, and spinach) and compared with real-time quantitative PCR (qPCR). No false-positive or false-negative results were observed among 120 bacterial strains used to evaluate assay specificity. The limits of detection of various STEC strains belonging to these target serogroups were approximately 1 to 20 CFU/reaction mixture in pure culture and 10(3) to 10(4) CFU/g in spiked food samples, which were comparable to those of qPCR. Standard curves generated suggested good linear relationships between STEC cell numbers and LAMP turbidity signals. In various beef and produce samples spiked with two low levels (1 to 2 and 10 to 20 CFU/25 g) of respective STEC strains, the LAMP assays consistently achieved accurate detection after 6 to 8 h of enrichment. ...Continue Reading

References

Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Nov 16, 2001·Biochemical and Biophysical Research Communications·Y MoriT Notomi
Aug 7, 2003·Applied and Environmental Microbiology·Fumito MaruyamaMasao Nasu
Sep 20, 2005·The Journal of Infectious Diseases·John T BrooksNancy A Strockbine
Sep 19, 2006·Applied and Environmental Microbiology·L M HallG J Gunn
Oct 3, 2006·Journal of Biochemical and Biophysical Methods·Hisatoshi KanekoTatsuo Suzutani
Nov 7, 2006·Journal of Animal Science·C L Gyles
Nov 17, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kristine E JohnsonCynthia L Sears
Feb 23, 2007·Journal of Medical Microbiology·Yukiko Hara-KudoMasanari Ikedo
Dec 26, 2007·International Journal of Food Microbiology·Yukiko Hara-KudoKosuke Takatori
Feb 12, 2008·Foodborne Pathogens and Disease·Yukiko Hara-KudoKosuke Takatori
Jun 14, 2008·Journal of Clinical Microbiology·Joshua HillAbhay Vats
Apr 28, 2009·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Yasuyoshi Mori, Tsugunori Notomi
Jun 12, 2010·Journal of Food Protection·Eiji YokoyamaKenitiro Ito
Jun 30, 2010·International Journal of Food Microbiology·Feifei Han, Beilei Ge
Aug 14, 2010·Foodborne Pathogens and Disease·Kayoko OhtsukaYukiko Hara-Kudo
Jan 5, 2011·Emerging Infectious Diseases·Elaine ScallanPatricia M Griffin
Feb 1, 2011·FEMS Immunology and Medical Microbiology·Patrice FrancoisJacques Schrenzel
Oct 28, 2011·The New England Journal of Medicine·Udo BuchholzMichael Kühne

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Citations

Feb 26, 2013·Journal of Agricultural and Food Chemistry·Felix FockeMarkus Fischer
Jun 13, 2013·Foodborne Pathogens and Disease·Fei WangBeilei Ge
May 29, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Deguo WangYanhong Liu
Jul 25, 2015·Scientific Reports·Xuzhi ZhangJ Justin Gooding
May 14, 2014·Letters in Applied Microbiology·T KuchtaD De Medici
Mar 31, 2015·Frontiers in Cellular and Infection Microbiology·Keri N NormanJames L Bono
Jun 16, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tiantian WangSanghyo Kim
Jun 21, 2014·Biosensors & Bioelectronics·Xuzhi ZhangJohn Justin Gooding
Oct 27, 2017·Current Medicinal Chemistry·Agnieszka ChylewskaMariusz Makowski
Oct 12, 2013·Journal of Food Protection·Qianru YangBeilei Ge
Jan 1, 2012·ISRN Bioinformatics·Nelson R Salinas, Damon P Little
Aug 5, 2017·Rapid Communications in Mass Spectrometry : RCM·Haorong Li, Jiangjiang Zhu
Jun 25, 2015·Microbiology Spectrum·Lothar Beutin, Patrick Fach
Jul 30, 2014·Analytical Chemistry·Carlos Duarte-GuevaraRashid Bashir

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