Rapid and specific detection of F17-related pilin and adhesin genes in diarrheic and septicemic Escherichia coli strains by multiplex PCR.

Journal of Clinical Microbiology
Y BertinJ P Girardeau

Abstract

The F17-related adhesins are prevalent in Escherichia coli strains isolated from calves with diarrhea or septicemia and from lambs with nephropathy. The F17 family includes the F17a, F17b, F17c, and F111 fimbriae produced by bovine E. coli strains and the G agglutinin produced by human uropathogenic E. coli strains. An easy and inexpensive multiplex PCR method was developed to detect all the F17-related fimbriae and to identify four subtypes of structural subunit genes and two distinct subfamilies of adhesin genes by only two runs of amplification. A strict correlation was observed between the phenotypic assays and the multiplex PCR method when 166 pathogenic E. coli strains isolated from intestinal content of calves or lambs were tested. Genes encoding the F17c structural subunit and the subfamily II adhesins were prominent among the bovine and ovine isolates, and the capsule-like CS31A antigen was strictly associated with the F17c fimbriae. The F17b subtype fimbriae were prominent among the bovine isolates producing the CNF2 toxin, whereas the F17a subtype fimbriae were associated with the bovine isolates producing neither the CS31A antigen nor the CNF2 toxin. Five bacterial strains possessed two distinct and complete F17-rel...Continue Reading

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Citations

Dec 11, 2008·Tropical Animal Health and Production·Reza Ghanbarpour, Eric Oswald
Jan 27, 2004·Infection and Immunity·Carol L PickettNancy A Strockbine
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Timothy J Johnson, Lisa K Nolan
Apr 21, 2005·Critical Reviews in Microbiology·K G MaciorowskiS C Ricke
Mar 20, 2013·Infection and Immunity·Ragnhild J BagerAnders M Bojesen
Sep 14, 2014·Applied and Environmental Microbiology·Charlotte ValatJean-Yves Madec
May 2, 2015·Polish Journal of Veterinary Sciences·M BednarskiP Sobiech
Mar 15, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Winston E MorrisMariano E Fernandez-Miyakawa
Sep 6, 2003·Journal of Clinical Microbiology·Sigrid Van BostJacques Mainil
Oct 14, 2016·EcoSal Plus·J Daniel DubreuilDieter M Schifferli
Dec 10, 2003·Journal of Clinical Microbiology·James R JohnsonThomas A Russo

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