Rapid apolipoprotein E genotyping from mailed buccal swabs

Journal of Neuroscience Methods
E IlveskoskiP J Karhunen

Abstract

Genetic polymorphism of apolipoprotein E (apoE) is linked with the risk of cardiovascular and Alzheimer's disease (AD). We determined apoE genotypes from mailed buccal cell swabs in 36 healthy individuals and 18 demented patients suffering from AD. DNA was released from the swabs by a rapid lysis technique. Polymerase chain reaction using 'hot start' and restriction enzyme digestion by HhaI was used for genotyping of apoE alleles, which were detected by polyacrylamide or MetaPhor agarose gel. ApoE genotypes determined from buccal cells or from stored leucocytes were identical to previously done phenotyping. An advantage of the present simplified buccal cell collection procedure is the possibility to collect DNA samples for apoE genotyping rapidly by mail from patients visiting their physician geographically distant from research laboratory. Moreover, venepuncture and storing of the samples in refrigerators are avoided and costs are reduced. This non-invasive and painless method is also applicable to other genetic screening tests in risk populations.

References

May 15, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·D M ThomsonA E Clague
Jan 1, 1988·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J DavignonC F Sing
Jan 31, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·P S HansenO Faergeman
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W J StrittmatterA D Roses
Oct 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·P W WilsonJ M Ordovas

❮ Previous
Next ❯

Citations

Jan 11, 2002·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·A Chaudhuri, P O Behan
Oct 18, 2008·Behavioural Brain Research·Laura H P EggermontErik J A Scherder
Oct 7, 2006·Acta Neurologica Scandinavica·A-M LoukoT Niskakangas
Aug 25, 2005·Scandinavian Cardiovascular Journal : SCJ·Pekka KuukasjärviPekka J Karhunen
Sep 6, 2001·Stroke; a Journal of Cerebral Circulation·T NiskakangasP J Karhunen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: Genetics

Alzheimer's disease is a neurodegenerative disease. Discover genetic and epigenetic aspects of Alzheimer’s disease, including genetic markers and genomic structural variations with this feed.

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.

Alzheimer's Disease: APOE

Apolipoprotein E (APOE) polymorphic alleles are major genetic risk factors for Alzheimer's disease. Discover the latest research on APOE and other genetic determinants of Alzheimer's disease here.