Rapid assessment of ocular drug delivery in a novel ex vivo corneal model.

Scientific Reports
Ghazala BegumRichard J Blanch

Abstract

Drug delivery by topical application has higher patient acceptance and lower morbidity than intraocular injection, but many ophthalmic treatments are unable to enter the eye or reach the posterior segment after topical application. The first stage towards posterior segment delivery after topical application is ocular surface penetration and existing models are in vivo or use large quantities of tissue. We therefore developed a novel ex vivo model using discs of porcine and human cornea and sclera (5 mm diameter) to assess penetration of a candidate neuroprotective siRNA. siRNA against caspase 2 or control solutions of known penetrance were applied to the corneal epithelial surface and trans-corneal penetration and corneal adsorbance measured at fixed time points. To demonstrate that leakage did not occur, we applied dextran blue, which should not penetrate the intact cornea and did not do so in our model. Fluorescein penetration (0.09%) was less than rhodamine B (6.98%) at 60 min. siCASP2 penetration was 0.01% by 60 min. When the applied siCASP2 was washed off after 2 min, (representing lacrimal drainage) 0.071% penetrated porcine cornea by 60 min and 0.0002% penetrated human cornea and 0.001% penetrated human sclera. Our ex vi...Continue Reading

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Citations

Jun 27, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Reshma Vasantha RamachandranAmbarish Ghosh
Oct 2, 2021·Drug Delivery·Ghazala BegumRichard James Blanch

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Methods Mentioned

BETA
biopsy

Clinical Trials Mentioned

NCT02341560

Software Mentioned

SPSS
Axiovision

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