Rapid assessment of the physiological status of the polychlorinated biphenyl degrader Comamonas testosteroni TK102 by flow cytometry.

Applied and Environmental Microbiology
Yoshinori Hiraoka, Kazuhide Kimbara

Abstract

The viability of the polychlorinated biphenyl-degrading bacterium Comamonas testosteroni TK102 was assessed by flow cytometry (FCM) with the fluorogenic ester Calcein-AM (CAM) and the nucleic acid dye propidium iodide (PI). CAM stained live cells, whereas PI stained dead cells. When double staining with CAM and PI was performed, three physiological states, i.e., live (calcein positive, PI negative), dead (calcein negative, PI positive), and permeabilized (calcein positive, PI positive), were detected. To evaluate the reliability of this double-staining method, suspensions of live and dead cells were mixed in various proportions and analyzed by FCM. The proportion of dead cells measured by FCM directly correlated with the proportion of dead cells in the sample (y = 0.9872 x + 0.18; R(2) = 0.9971). In addition, the proportion of live cells measured by FCM inversely correlated with the proportion of dead cells in the sample (y = -0.9776 x + 98.36; R(2) = 0.9962). The proportion of permeabilized cells was consistently less than 2%. These results indicate that FCM in combination with CAM and PI staining is rapid (<or=1 h) and distinguishes correctly among live, dead, and permeabilized cells.

References

Aug 1, 1979·Applied and Environmental Microbiology·K FurukawaA Kamibayashi
Nov 1, 1992·Applied Microbiology and Biotechnology·J P DiaperC Edwards
Jul 1, 1990·The Journal of Applied Bacteriology·A C PinderD C Clark
May 1, 1987·Applied and Environmental Microbiology·D L BedardM J Brennan
Jan 1, 1973·Canadian Journal of Microbiology·M Ahmed, D D Focht
Jul 1, 1984·Antimicrobial Agents and Chemotherapy·R E Hancock, P G Wong
Oct 1, 1995·The Journal of Applied Bacteriology·J PorterR W Pickup
Nov 1, 1994·The Journal of Antimicrobial Chemotherapy·R S Pore
Dec 1, 1995·International Journal of Food Microbiology·J UeckertP F ter Steeg
Jul 2, 1998·Applied and Environmental Microbiology·P LebaronN Parthuisot
Aug 26, 1998·Journal of Applied Microbiology·G N CaronR A Badley
Mar 4, 2000·Applied and Environmental Microbiology·T S GunasekeraD A Veal
Mar 29, 2000·Journal of Applied Microbiology·I M Helander, T Mattila-Sandholm
Jul 1, 1995·Applied and Environmental Microbiology·R I JeprasM J Wilkinson
Jul 1, 1997·Applied and Environmental Microbiology·F JouxM Troussellier

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Citations

May 31, 2006·Applied Microbiology and Biotechnology·T YamadaK Kimbara
Jul 10, 2003·Current Opinion in Biotechnology·Kazuya Watanabe, Natsuko Hamamura
May 5, 2006·Applied and Environmental Microbiology·Militza Carrero-ColónAllan Konopka
Sep 26, 2013·BioMed Research International·Mónica I Villaseñor-CardosoEnrique Ortega
May 31, 2011·Journal of Immunological Methods·Agnieszka RobaszkiewiczMiroslaw Soszynski
Dec 5, 2015·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Liping QiuXuntao Wang
Mar 4, 2010·Acta Biomaterialia·Alicia Calzado-MartínNuria Vilaboa
Jan 4, 2012·Journal of Biomedical Materials Research. Part a·Baichuan WangYannan Zhang
Aug 29, 2006·International Journal of Food Microbiology·Konstantinos PapadimitriouEffie Tsakalidou
Mar 27, 2010·FEMS Microbiology Reviews·Susann Müller, Gerhard Nebe-von-Caron
Mar 8, 2019·Microbiology·Shravanthi S Kumar, Asit Ranjan Ghosh
Apr 27, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Steven L PercivalJohn G Thomas
Dec 3, 2008·Journal of Food Protection·Ultan P Cronin, Martin G Wilkinson
Apr 4, 2021·Cells·Agnieszka KaminskaAnna Sarnowska

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