Rapid assessment of viable but non-culturable Bacillus coagulans MTCC 5856 in commercial formulations using Flow cytometry

PloS One
Muhammed MajeedLakshmi Mundkur

Abstract

Accurate enumeration of bacterial count in probiotic formulation is imperative to ensure that the product adheres to regulatory standards and citation in consumer product label. Standard methods like plate count, can enumerate only replicating bacterial population under selected culture conditions. Viable but non culturable bacteria (VBNC) retain characteristics of living cells and can regain cultivability by a process known as resuscitation. This is a protective mechanism adapted by bacteria to evade stressful environmental conditions. B. coagulans MTCC 5856(LactoSpore®) is a probiotic endospore which can survive for decades in hostile environments without dividing. In the present study, we explored the use of flow cytometry to enumerate the viable count of B. coagulans MTCC 5856 under acidic and alkaline conditions, high temperature and in commercial formulations like compressed tablets and capsules. Flow cytometry (FCM) was comparable to plate count method when the spores were counted at physiological conditions. We show that VBNC state is induced in B. coagulans MTCC 5856by high temperature and acidic pH. The cells get resuscitated under physiological conditions and FCM was sensitive to detect the VBNC spores. Flow cytometr...Continue Reading

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Citations

Sep 26, 2020·Pharmaceutics·Jordan PascoeJoanna Bacon
Dec 16, 2020·Comprehensive Reviews in Food Science and Food Safety·Kai DongXiaojun Liao
Apr 6, 2021·Critical Reviews in Food Science and Nutrition·Vincenzina FuscoDaniele Chieffi

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

GraphPad
Graph Pad prism
Diva

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