Rapid authentication of animal cell lines using pyrolysis mass spectrometry and auto-associative artificial neural networks.

Cytotechnology
R GoodacreD B Kell

Abstract

Pyrolysis mass spectrometry (PyMS) was used to produce biochemical fingerprints from replicate frozen cell cultures of mouse macrophage hybridoma 2C11-12, human leukaemia K562, baby hamster kidney BHK 21/C13, and mouse tumour BW-O, and a fresh culture of Chinese hamster ovary CHO cells. The dimensionality of these data was reduced by the unsupervised feature extraction pattern recognition technique of auto-associative neural networks. The clusters observed were compared with the groups obtained from the more conventional statistical approaches of hierarchical cluster analysis. It was observed that frozen and fresh cell line cultures gave very different pyrolysis mass spectra. When only the frozen animal cells were analysed by PyMS, auto-associative artificial neural networks (ANNs) were employed to discriminate between them successfully. Furthermore, very similar classifications were observed when the same spectral data were analysed using hierarchical cluster analysis. We demonstrate that this approach can detect the contamination of cell lines with low numbers of bacteria and fungi; this approach could plausibly be extended for the rapid detection of mycoplasma infection in animal cell lines. The major advantages that PyMS of...Continue Reading

References

Apr 27, 1992·Journal of Immunological Methods·C C UphoffH G Drexler
Mar 1, 1990·Cytotechnology·J M Mowles, A Doyle
Jan 1, 1992·Journal of Biotechnology·R G WernerA Konrad
Apr 27, 1992·Journal of Immunological Methods·C C UphoffH G Drexler
Sep 1, 1990·FEMS Microbiology Letters·R Goodacre, R C Berkeley
Jun 1, 1988·Analytical Biochemistry·R J Hay
Nov 15, 1984·International Journal of Cancer. Journal International Du Cancer·P De BaetselierM Feldman
Jul 28, 1995·International Journal of Cancer. Journal International Du Cancer·J G ParkK U Lee
Feb 1, 1995·In Vitro Cellular & Developmental Biology. Animal·K G SteubeH G Drexler
Aug 1, 1994·Trends in Biotechnology·G Montague, J Morris
Oct 1, 1993·In Vitro Cellular & Developmental Biology. Animal·A HopertH G Drexler
Feb 1, 1996·Current Opinion in Biotechnology·R Goodacre, D B Kell
Aug 1, 1996·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·R GoodacreM J Neal
Aug 1, 1996·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·R GoodacreD B Kell
Jan 15, 1996·Analytical Chemistry·R Goodacre, D B Kell

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Citations

May 17, 2005·Journal of Industrial Microbiology & Biotechnology·Mariët J van der WerfThomas Hankemeier
Oct 20, 2019·Metabolomics : Official Journal of the Metabolomic Society·Kevin M MendezStacey N Reinke

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