Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry

Scientific Reports
Yannick CharretierJean-Philippe Charrier

Abstract

Mass spectrometry (MS) in Selected Reaction Monitoring (SRM) mode is proposed for in-depth characterisation of microorganisms in a multiplexed analysis. Within 60-80 minutes, the SRM method performs microbial identification (I), antibiotic-resistance detection (R), virulence assessment (V) and it provides epidemiological typing information (T). This SRM application is illustrated by the analysis of the human pathogen Staphylococcus aureus, demonstrating its promise for rapid characterisation of bacteria from positive blood cultures of sepsis patients.

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Citations

Mar 10, 2016·Journal of Mass Spectrometry : JMS·Elodie DuriezEric Ezan
Jun 18, 2016·Proteomics. Clinical Applications·Yannick Charretier, Jacques Schrenzel
Jul 18, 2019·Expert Review of Proteomics·Martin WelkerDavid Pincus
Jan 17, 2020·Molecular & Cellular Proteomics : MCP·Roger KarlssonEdward R B Moore
Jun 2, 2017·Scientific Reports·Honghui WangJohn P Dekker
Jul 17, 2018·Frontiers in Microbiology·Alex van Belkum, Olivier Rochas
Jun 27, 2019·Proteomics·Sara SalehKris Gevaert
Jan 20, 2020·World Journal of Microbiology & Biotechnology·Sujintana WongthongAroonlug Lulitanond
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Feb 15, 2020·Nature Reviews. Microbiology·Alex van BelkumWilliam Michael Dunne
Sep 27, 2019·Frontiers in Microbiology·Karim HayounBéatrice Alpha-Bazin
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May 22, 2020·Clinical Proteomics·Fumio NomuraKazuyuki Matsushita
Jan 17, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·K S JangJ S Kim
Sep 4, 2020·Journal of Clinical Microbiology·Honghui WangJohn P Dekker
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Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Roger KarlssonEdward R B Moore
Sep 16, 2020·Journal of Proteome Research·Mathieu DupréJulia Chamot-Rooke
Oct 27, 2021·Analytical Chemistry·Christian BlumenscheitJoerg Doellinger

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Methods Mentioned

BETA
PCR
ELISA

Software Mentioned

Analyst
MultiQuant TM

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