Rapid Bactericidal Action of Propolis against Porphyromonas gingivalis

Journal of Dental Research
Y YoshimasuR Nakao

Abstract

Propolis, a resinous substance produced by bees, is used as a folk medicine for treatment of periodontal diseases. However, its mode of the action and the compounds responsible for its activities remain obscure. In the present study, we comprehensively investigated the antibacterial activities of ethanol-extracted propolis (EEP) and EEP-derived compounds toward Porphyromonas gingivalis, a keystone pathogen for periodontal diseases. Broth microdilution and agar dilution assays were used to determine the minimum inhibitory concentrations of EEP against a range of oral bacterial species, of which P. gingivalis showed a higher level of sensitivity than oral commensals such as streptococci. Its antibacterial activity toward P. gingivalis was maintained even after extensive heat treatment, demonstrating a high level of thermostability. EEP also induced death of P. gingivalis cells by increasing membrane permeability within 30 min. Spatiotemporal analysis based on high-speed atomic force microscopy revealed that EEP immediately triggered development of aberrant membrane blebs, followed by bleb fusion events on the bacterial surface. Furthermore, we isolated artepillin C, baccharin, and ursolic acid from EEP as antibacterial compounds ...Continue Reading

References

Mar 12, 1998·Journal of Clinical Periodontology·S S SocranskyR L Kent
Jul 3, 1998·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G A Burdock
Oct 26, 1999·Infection and Immunity·B R DornA Progulske-Fox
Apr 20, 2000·The Journal of Clinical Investigation·E LallaA M Schmidt
Apr 18, 2002·Journal of Agricultural and Food Chemistry·Yong K ParkClaudio L Aguiar
May 26, 2005·Journal of Ethnopharmacology·Mitsue F HayacibaraJaime A Cury
Jul 28, 2010·Antimicrobial Agents and Chemotherapy·Jie Wu, Hua Xie
Sep 4, 2012·Nature Reviews. Microbiology·George HajishengallisMichael A Curtis
Sep 5, 2012·Indian Journal of Dental Research : Official Publication of Indian Society for Dental Research·Amita Coutinho
Nov 6, 2012·Research in Microbiology·Ian A MacDonald, Meta J Kuehn
Aug 15, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Robert D WojtyczkaTomasz J Wąsik
Nov 29, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shuai HuangFu-Liang Hu
Dec 3, 2014·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Aiko YoshidaKunio Takeyasu
Apr 12, 2015·Microbes and Infection·Nicholas S Jakubovics, J Grant Burgess
Nov 28, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Łukasz WoźniakKrystian Marszałek
Mar 19, 2016·Phytotherapy Research : PTR·José M Sforcin
Nov 30, 2016·Scientific Reports·Esteban Rodriguez HerreroWim Teughels
Jun 14, 2017·Antimicrobial Agents and Chemotherapy·Andreas BauwensMartina Bielaszewska
Jul 28, 2017·Journal of Oral Microbiology·Hwei Sze OngEric C Reynolds

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Citations

Apr 25, 2019·Biofouling·Stela Lima Farias MirandaBruno Bueno-Silva
May 31, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Izabela Przybyłek, Tomasz M Karpiński
Mar 1, 2020·Odontology·Ryoma NakaoYorimasa Ogata
Dec 25, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Anupon IadnutKhajornsak Tragoolpua
Jan 24, 2021·Journal of Oral Biosciences·Masae FurukawaKenji Matsushita
Jul 3, 2021·Antibiotics·Rita Abou NaderZiad Fajloun
Aug 28, 2021·Nutrients·Priyanshu BhargavaSunil C Kaul

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Methods Mentioned

BETA
scanning
transmission
flow cytometry
atomic force microscopy
fluorescence spectrometry
transmission electron microscopy
fluorescence-activated cell sorting

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