Rapid bidirectional allele-specific PCR identification for triazine resistance in higher plants

Pest Management Science
Xin Tian, Henri Darmency

Abstract

Triazine resistance is reported to be due to chloroplast herbicide target insensitivity in most species, and this is most often caused by a Ser(264)-Gly mutation at the D1 protein. In order to ascertain whether this mutation is really predominant amongst resistant plants, and also for gene flow studies, a rapid test is needed that allows the testing of large quantities of plants. Here a bidirectional allele-specific PCR (polymerase chain reaction) identification is proposed. The designed primers were shown to be universal in the three grass and three broadleaf species examined.

References

May 1, 1990·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·D NaberJ J van Rensen
Oct 1, 1988·Molecular & General Genetics : MGG·F SatoY Yamada
Feb 22, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R D RiosA R Prina
Sep 27, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T WangJ Gressel
Nov 9, 2004·Plant Physiology·Christophe DélyeHenri Darmency
Dec 23, 1983·Science·J Hirschberg, L McIntosh
Aug 1, 1999·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J E FreyD Frei
Aug 1, 1988·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J E Thomzik, R Hain
Jan 1, 1987·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M Reith, N A Straus

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