Rapid bioassay to measure early reactive oxygen species production in Arabidopsis leave tissue in response to living Pseudomonas syringae

Plant Methods
John M Smith, Antje Heese

Abstract

Arabidopsis thaliana and Pseudomonas syringae pathovar tomato (Pto) provide an excellent plant-bacteria model system to study innate immunity. During pattern-triggered immunity (PTI), cognate host receptors perceive pathogen-associated molecular patterns (PAMPs) as non-self molecules. Pto harbors many PAMPs; thus for experimental ease, many studies utilize single synthesized PAMPs such as flg22, a short protein peptide derived from Pseudomonas flagellin. Flg22 recognition by Arabidopsis Flagellin Sensing 2 (FLS2) initiates a plethora of signaling responses including rapid production of apoplastic reactive oxygen species (ROS). Assessing flg22-ROS has been instrumental in identifying novel PAMP-signaling components; but comparably little is known whether in Arabidopsis, ROS is produced in response to intact live Pto and whether this response can be used to dissect genetic requirements of the plant host and live bacterial pathogens in planta. Here, we report of a fast and robust bioassay to quantitatively assess early ROS in Arabidopsis leaves, a tissue commonly used for pathogen infection assays, in response to living bacterial Pto strains. We establish that live Pto elicits a transient and dose-dependent ROS that differed in ti...Continue Reading

References

Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·G FelixT Boller
Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·L Gómez-GómezT Boller
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Miguel Angel TorresJonathan D G Jones
Mar 8, 2003·Microbiology·Sonia L BardyKen F Jarrell
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon
Jul 13, 2007·Nature·Delphine ChinchillaThomas Boller
Jul 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Antje HeeseJohn P Rathjen
Jul 27, 2007·The Plant Journal : for Cell and Molecular Biology·Thomas S NühseScott C Peck
Oct 10, 2009·Plant Signaling & Behavior·Selena Gimenez-IbanezJohn P Rathjen
Dec 17, 2009·Physiologia Plantarum·Miguel Angel Torres
Jan 28, 2010·The Journal of Biological Chemistry·Birgit SchulzeDelphine Chinchilla
May 18, 2010·The Journal of Biological Chemistry·David A KorasickAntje Heese
Jul 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Freddy BoutrotJohn P Rathjen
Feb 22, 2012·Trends in Microbiology·Magdalen LindebergAlan Collmer
May 15, 2012·Trends in Plant Science·Andrea A GustThorsten Nürnberger
Jun 19, 2012·Current Opinion in Plant Biology·Jacqueline Monaghan, Cyril Zipfel

❮ Previous
Next ❯

Citations

Jun 26, 2015·Journal of Integrative Plant Biology·Marta BjornsonKatayoon Dehesh
Jun 14, 2015·Biochemical and Biophysical Research Communications·Tian WeiHongxia Liu
Mar 2, 2016·Molecular Plant-microbe Interactions : MPMI·Ofir BaharPamela C Ronald
Mar 7, 2015·Molecular Plant·Jean BigeardHeribert Hirt
Jun 30, 2015·Developmental Cell·Jeffrey L CaplanSavithramma P Dinesh-Kumar
Apr 7, 2017·Molecular Plant-microbe Interactions : MPMI·Suma ChakravarthyMelanie J Filiatrault
Nov 25, 2017·Molecular Plant-microbe Interactions : MPMI·Kaixuan DuanZhanyuan J Zhang
Jun 11, 2017·Molecular Plant Pathology·Min Woo LeeFiona L Goggin
Sep 3, 2019·EMBO Reports·Yunhe JiangHeribert Hirt
Dec 6, 2019·Scientific Reports·Linda NietzschmannSabine Rosahl
Feb 9, 2017·Frontiers in Plant Science·Natalia MorozKiwamu Tanaka
Feb 23, 2020·Frontiers in Plant Science·Cristián Jacob, Maeli Melotto
Jul 2, 2020·International Journal of Molecular Sciences·Yanping JingSheng Luan
Oct 28, 2017·Frontiers in Plant Science·Christian E LammUwe Sonnewald
Nov 25, 2020·Proceedings of the National Academy of Sciences of the United States of America·Adam D SteinbrennerEric A Schmelz
Oct 31, 2020·The Plant Journal : for Cell and Molecular Biology·Pierre BuscaillRenier A L van der Hoorn
Dec 22, 2020·Critical Reviews in Plant Sciences·Pai LiBrad Day
Mar 7, 2021·Proceedings of the National Academy of Sciences of the United States of America·Ángela RománMichael J Haydon
Mar 16, 2021·Frontiers in Immunology·Edgar Ramos-MartínezMarco V José
Apr 28, 2021·Plant Science : an International Journal of Experimental Plant Biology·Lisa HeymanMonica Höfte
Sep 26, 2021·Proceedings of the National Academy of Sciences of the United States of America·Romain SchellenbergerStéphan Dorey

❮ Previous
Next ❯

Software Mentioned

Graph Pad Prism4

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.