Rapid biochemical characterization of Haemophilus species by using the micro-ID.

Journal of Clinical Microbiology
S C EdbergJ M Singer

Abstract

Biotyping of Haemophilus influenzae into five type and H. parainfluenzae into three types based on indole production, ornithine decarboxylase, and urease has been reported (M. Kilian, Acta Pathol. Microbiol. Scand. Sect. B 82:835--842, 1976). A commercially available test system designed for the 4-h identification of Enterobacteriaceae. Micro-ID, proved efficacious for the rapid biotyping of these two Haemophilus species. The nitrate reductase, indole production, ornithine decarboxylase, urease, and o-nitrophenyl-beta-D-galactopyranoside hydrolysis tests in Micro-ID correlated over 99% with conventional methodology. By utilizing the indole and o-nitrophenyl-beta-D-galactopyranoside tests it was possible, with 261 of 272 (96.1%) isolates, to distinguish H. influenzae from H. parainfluenzae. Cerebrospinal fluid isolates were over 90% H. influenzae biotype I, and conjunctival isolates were approximately 70% biotype II. Type b H. influenzae were predominantly biotypes I and II; these type b isolates were also overwhelmingly indole producers. Although over 90% of biotypes I and II have been reported to produce beta-lactamase, this was not confirmed by the small number of beta-lactamase producers encountered here. The 4-h Micro-ID sh...Continue Reading

References

Mar 1, 1979·Journal of Clinical Microbiology·M KilianW Frederiksen
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Mar 1, 1978·Journal of Clinical Microbiology·A E Back, T R Oberhofer
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Jan 1, 1955·Acta Pathologica Et Microbiologica Scandinavica·V MØLLER
Feb 1, 1952·Journal of General Microbiology·P H CLARKE, S T COWAN
Nov 1, 1974·Antimicrobial Agents and Chemotherapy·C Thornsberry, L A Kirven

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Citations

Jun 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R QuentinA Goudeau
Jan 1, 1983·Journal of Clinical Pathology·S Mehtar, S A Afshar
Oct 1, 1985·Journal of Clinical Pathology·B Holmes, P S Humphry
Jul 1, 1990·Journal of Clinical Pathology·P G MurphyE T Smyth
Sep 1, 1983·Journal of Clinical Microbiology·J S MatthewsA L Jenkins
Nov 1, 1983·Journal of Clinical Microbiology·L A Eriquez, N E Hodinka
Sep 1, 1983·Journal of Clinical Microbiology·M J Chang, G Controni
Oct 19, 2007·Journal of Clinical Microbiology·Erik L Munson, Gary V Doern
Jan 1, 1987·Journal of Clinical Microbiology·W M JandaP C Schreckenberger
Feb 1, 1982·Journal of Clinical Microbiology·D F WelchJ M Matsen
Jun 1, 1982·Journal of Clinical Microbiology·R L HolmesM Otto
Mar 1, 1983·Journal of Clinical Microbiology·P J MalloyP C Schreckenberger

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