Rapid bone regeneration by Escherichia coli-derived recombinant human bone morphogenetic protein-2 loaded on a hydroxyapatite carrier in the rabbit calvarial defect model

Biomaterials Research
Chung-Hoon ChungSeong-Ho Choi

Abstract

The aim of this study was to determine the osteoconductivity of hydroxyapatite particles (HAP) as a carrier for Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2). Two 8-mm diameter bicortical calvarial defects were created in each of 20 rabbits. One of each pair of defects was randomly assigned to be filled with HAP only (HAP group) or ErhBMP-2 loaded HAP (ErhBMP-2/HAP group), while the other defect was left untreated (control group). The animals were killed after either 2 weeks (n = 10) or 8 weeks (n = 10) of healing, and histological, histomorphometric, and tomographic analyses were performed. All experimental sites showed uneventful healing during the postoperative healing period. In both histomorphometric and tomographic analyses, the new bone area or volume of the ErhBMP-2/HAP group was significantly greater than that of the HAP and control groups at 2 weeks (p < 0.05). However, at 8 weeks, no significant difference in new bone area or volume was observed between the ErhBMP-2/HAP and HAP groups. The total augmented area or volume was not significantly different between the ErhBMP-2/HAP and HAP groups at 2 and 8 weeks. Combining ErhBMP-2 with HAP could significantly promote rapid initial new...Continue Reading

References

Oct 16, 1999·Clinical Oral Implants Research·K BesshoJ L Ong
Sep 3, 2002·Spine·John M Wozney
Feb 16, 2007·International Journal of Oral and Maxillofacial Surgery·J LanX R Cheng
Feb 20, 2007·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Dritan TurhaniRolf Ewers
Mar 22, 2008·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Alan S Herford, Philip J Boyne
Dec 17, 2009·The Spine Journal : Official Journal of the North American Spine Society·Harel Deutsch
May 1, 2010·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Weimin ZhuYanjun Zeng
Sep 30, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Jee-Hee JungKyoo-Sung Cho
Oct 26, 2011·Acta Biomaterialia·Antonio HernándezCarmen Évora
Nov 19, 2011·Clinical Oral Implants Research·P R SchmidlinF E Weber
Jun 9, 2012·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Zhi-qing JiangDe-zhi Shang
Jan 15, 2014·The Spine Journal : Official Journal of the North American Spine Society·Chadi A Tannoury, Howard S An
Jan 16, 2014·Tissue Engineering. Part C, Methods·Teja GudaPamela R Brown Baer
Dec 3, 2014·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Pil-Young YunYeon-Jo Choi
May 9, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Zhiwei LuHanbing Rao

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
xenograft
PCR
enzyme-linked immunosorbent assay

Software Mentioned

Pro Plus
Image
SPSS

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.