PMID: 9553870Apr 29, 1998Paper

Rapid chair-side DNA probe assay of Bacteroides forsythus and Porphyromonas gingivalis

Journal of Periodontal Research
A C R TannerR L Kent

Abstract

This study compared a rapid, colorimetric DNA probe assay designed to be performed in a dental office within 40 min, with anaerobic culture and indirect immunofluorescence microscopy (IFM) for detection of Bacteroides forsythus and Porphyromonas gingivalis in subgingival plaque samples. The DNA probe assay used the Periodontal Microbial Identification Test (Saigene Corporation, Bothell, Washington, USA). B. forsythus was detected in 46 (52%), 49 (55%) and 39 (44%) of the samples by DNA probe, culture (at levels > or = 10(5)) and IFM, respectively. P. gingivalis was detected in 24 (27%), 18 (20%) and 29 (33%) of the samples by DNA probe, culture (at levels > or = 10(5)) and IFM, respectively. Results from the DNA probe assay were compared to culture. Culture negative, probe positive samples were re-evaluated by IFM, and IFM positive samples were considered positive in "resolved" data. Using resolved data. DNA probe detection sensitivity and specificity values for B. forsythus were 81% and 91% and for P. gingivalis were 80% and 95%, respectively. DNA probe test results were further compared with culture and IFM. For samples negative by both culture and IFM, probe specificity was 92% in 25 B. forsythus samples and 95% in 57 P. gin...Continue Reading

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Citations

Oct 5, 2001·Clinical Microbiology Reviews·W J Loesche, N S Grossman
Feb 5, 2002·Journal of Dental Research·A C R TannerJ Bruss
Oct 23, 2002·Journal of Clinical Periodontology·Andrea MombelliNiklaus P Lang
Apr 19, 2003·Journal of Clinical Periodontology·Ching Yin TsaiJim Hodges
Aug 1, 2003·Journal of Periodontology·UNKNOWN Research, Science and Therapy Committee
Jun 26, 2003·Oral Microbiology and Immunology·Y HuangI Ishikawa
Aug 13, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Georg Conrads
Mar 23, 2000·Journal of Dental Research·P J Macuch, A C Tanner
Aug 26, 2006·Periodontology 2000·Anne C R Tanner, Jacques Izard
Jul 4, 2013·Pathogens and Disease·Livia S FinotiRaquel M Scarel-Caminaga
Mar 14, 2007·Acta Odontologica Scandinavica·Nattaporn YouravongGunnar Dahlén
Feb 9, 2008·Critical Reviews in Microbiology·Simonetta D'ErcoleRaffaele Piccolomini
Oct 17, 2009·International Journal of Dental Hygiene·Maria Perno Goldie
Sep 30, 2003·Journal of Periodontology·Gary C Armitage, UNKNOWN Research, Science and Therapy Committee of the American Academy of Periodontology
Feb 21, 2003·FEMS Microbiology Letters·Joong Ki KookByung Moo Min
Jun 25, 2016·The Journal of Contemporary Dental Practice·Deema Farsi, Anne Tanner

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