PMID: 11607219Oct 1, 1991Paper

Rapid changes of protein phosphorylation are involved in transduction of the elicitor signal in plant cells

Proceedings of the National Academy of Sciences of the United States of America
G FelixThomas Boller

Abstract

Plant cells have an acute sense for pathogen-derived chemical stimuli, so-called elicitors, which induce the plant's defense response. To investigate the molecular basis of chemosensory transduction, elicitor-treated tomato cells were labeled with 1-min pulses of [32P] phosphate. This technique revealed drastic changes in protein phosphorylation in vivo within minutes of stimulation. The protein kinase inhibitors K-252a and staurosporine completely prevented these elicitor-induced changes in protein phosphorylation. They also blocked two early biochemical responses to elicitors, extracellular alkalinization and biosynthesis of ethylene. The ability of K-252a, staurosporine, and benzoylated staurosporine derivatives to inhibit elicitor responses in vivo correlated with their ability to inhibit tomato microsomal protein kinase in vitro. When K-252a was given to elicited cells 1 min after the[32] phosphate, the radioactivity in certain newly labeled phosphoprotein bands disappeared again within minutes. This correlated with an arrest of alkalinization within minutes when K-252a was applied in midcourse of elicitation. These data show that phosphorylation of protein substrates by K-252a-sensitive protein kinases is essential for tr...Continue Reading

References

May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M A LawtonC J Lamb
Jun 1, 1989·Trends in Pharmacological Sciences·U T Rüegg, G M Burgess
Sep 11, 1987·Cell·T Hunter
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E E FarmerC A Ryan
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W E Schmidt, J Ebel

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Citations

May 1, 1992·Plant Molecular Biology·C A Ryan
Aug 1, 1993·Trends in Microbiology·W P LindsayR A Dixon
Mar 6, 1999·Current Opinion in Plant Biology·D Scheel
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·S ZhangD F Klessig
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Chandra, P S Low
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·H Sano, Y Ohashi
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·P ReymondE E Farmer
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·S UsamiY Machida
Nov 20, 2008·Plant & Cell Physiology·Bastiaan O R BargmannTeun Munnik
Jan 13, 2004·Molecular Plant-microbe Interactions : MPMI·Hubert H FelleKarl-Heinz Kogel
Aug 14, 2010·Journal of Molecular Signaling·Shilpa RamaniChelliah Jayabaskaran
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·S Zhang, D F Klessig
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·J StratmannC A Ryan
Mar 13, 2012·Annual Review of Plant Biology·Benjamin Schwessinger, Pamela C Ronald
Aug 12, 2000·Molecular Plant-microbe Interactions : MPMI·F Lecourieux-OuakedA Lebrun-Garcia
Jun 2, 2001·Molecular Plant-microbe Interactions : MPMI·H YoshiokaN Doke
Nov 20, 2012·Journal of Theoretical Biology·J A FozardM J Bennett
Feb 18, 2009·Plant, Cell & Environment·Bastiaan O R BargmannTeun Munnik
Nov 4, 2006·Cellular Microbiology·Sabine DesenderFlorence Val
Jul 27, 2007·The Plant Journal : for Cell and Molecular Biology·Thomas S NühseScott C Peck
Jul 27, 2006·The New Phytologist·David LecourieuxAlain Pugin
Jan 18, 2006·The Plant Journal : for Cell and Molecular Biology·Bastiaan O R BargmannTeun Munnik
Jul 22, 2014·Molecular Plant Pathology·Giovanni FaraceStéphan Dorey
Sep 8, 2015·Journal of Proteomics·Ján A MiernykMark L Johnston
Apr 26, 2005·Biotechnology Advances·Jian ZhaoRobert Verpoorte
Jun 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Erica J WashingtonJeffery L Dangl
Aug 12, 2016·Frontiers in Plant Science·Nicolas BensoussanVojislava Grbić

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