Rapid characterization of glycosaminoglycans using a combined approach by infrared and Raman microspectroscopies

Journal of Pharmaceutical Sciences
Nathalie MainreckYanusz Wegrowski

Abstract

This study describes a complementary infrared (IR) and Raman spectroscopic investigations of a set of biomolecule representatives of glycosaminoglycans (GAGs) class. Both IR and Raman data exhibit characteristic spectral signatures that allow a direct molecular distinction of these compounds. Comparison of these molecular signatures clearly evidences the differences between heparan sulfate and heparin by computing the intensity ratio between the 1248 cm(-1) and 1043 cm(-1) peaks, corresponding respectively to sulfate and C-O-C linkages. Identically, chondroitin-4-sulfate and chondroitin-6-sulfate are differentiated via the 730 cm(-1) and 853 cm(-1) bands (axial orientation) and the 822 cm(-1) and 1000 cm(-1) bands (equatorial orientation). These orientations concern the sulfate groups of these molecules. Secondly, multivariate statistical methods were employed to analyze the data set. Hierarchical cluster analysis (HCA) of both IR and Raman spectra showed a very good differentiation between the different structures. In addition, the sulfatation degree could be obtained from this classification. Principal component analysis using three principal components (PCs) confirmed the findings of HCA and, furthermore, comparisons of the ...Continue Reading

References

Jul 17, 1978·Biochimica Et Biophysica Acta·R BansilH E Stanley
Jan 1, 1991·Analytical Biochemistry·M O Longas, K O Breitweiser
Aug 1, 1993·Journal of Biomolecular Structure & Dynamics·S A LeeA Rupprecht
Jan 1, 1996·Journal of Pharmaceutical Sciences·D H AthaV Reipa
Feb 13, 2001·International Journal of Biological Macromolecules·R ServatyK Arnold
Jul 12, 2003·Journal of Biomolecular Structure & Dynamics·I ReineckA Rupprecht
Jun 1, 1954·Biochimica Et Biophysica Acta·S F ORR
Feb 8, 1958·Nature·M B MATHEWS
Feb 13, 2009·Connective Tissue Research·Richard EllisC Peter Winlove
Apr 25, 2009·Clinics in Podiatric Medicine and Surgery·Kirk A GroganRobert S Salk
May 6, 2009·Facial Plastic Surgery : FPS·Karen L BeasleyRobert A Weiss
May 21, 2009·Journal of Biomaterials Science. Polymer Edition·Yasuhiro MatsumotoYoshimitsu Kuroyanagi

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Citations

Nov 22, 2011·Analytical and Bioanalytical Chemistry·Maha A El-HagrasyEiichi Tamiya
Jul 16, 2014·Analytical and Bioanalytical Chemistry·Stéphane BrézillonGanesh D Sockalingum
Feb 6, 2013·Biochimica Et Biophysica Acta·Marie GuilbertGanesh D Sockalingum
Mar 14, 2017·Tissue Engineering. Part a·Gloria Portocarrero HuangTreena Livingston Arinzeh
Nov 8, 2018·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Aurélie VillaretGwendal Josse
Mar 23, 2019·Physical Chemistry Chemical Physics : PCCP·Václav ProfantVladimír Baumruk
Dec 9, 2016·Glycoconjugate Journal·Hossam Taha MohamedStéphane Brézillon
Jun 18, 2020·Frontiers in Cell and Developmental Biology·Stéphane BrézillonGanesh D Sockalingum
Sep 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hossam Taha MohamedStéphane Brézillon
Aug 18, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Natalia A BurmistrovaYulia B Monakhova
Dec 8, 2020·Journal of Pharmaceutical and Biomedical Analysis·Natalia A BurmistrovaYulia B Monakhova
Mar 18, 2019·Acta Biomaterialia·Roseline MenezesTreena L Arinzeh
Jun 8, 2021·Chemical Science·Miriam de J Velásquez-HernándezPaolo Falcaro
Sep 14, 2012·Analytical Chemistry·Roberta GalliMatthias Kirsch

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