Rapid characterization of plant mutants with an altered ion-profile: a case study using Lotus japonicus.

The New Phytologist
Zheng ChenMitsuru Osaki

Abstract

Legumes are second only to cereals in their importance to humans, and study of their functional genomics of nutrition and other trace elements is crucial for agricultural production and food fortification. We describe here an ionomic screening experiment carried out to investigate the accumulation of 15 elements in shoots of mutants of Lotus japonicus, a good genetic tool for legume study.Approximately 2000 mutagenized M2 plants were cultivated in a novel low-cost high-throughput system and their elemental profiles were determined by inductively coupled plasma mass spectroscopy (ICP-MS).After triple-checking the element concentrations in M2 or M3 plant shoots, 31 mutants with altered elemental profiles were identified. Surprisingly, the number of genes regulating essential elements was similar to the number regulating nonessential elements. Magnesium (Mg) and nickel (Ni) were correlated in a number of mutants.Further investigation suggested that phosphorus (P) and cobalt (Co) might be involved in the ion homeostasis network of Mg and Ni.The results suggested that the pathways for element uptake or translocation were highly linked through the ion transport-related genes. Ionomics proved to be a powerful functional genomics tool ...Continue Reading

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Citations

May 15, 2012·Analytical and Bioanalytical Chemistry·Francisco ArdiniMarco Grotti
Jan 19, 2010·Briefings in Functional Genomics·Ivan Baxter
Aug 14, 2009·Plant Physiology·Richard ThompsonKarine Gallardo
Jan 14, 2016·Biological Trace Element Research·Kyoung-Min SoMyung Il Chung
May 30, 2009·Current Opinion in Plant Biology·Elizabeth A H Pilon-SmitsMichela Schiavon
Dec 25, 2015·SpringerPlus·Toshihiro WatanabeMitsuru Osaki
Apr 23, 2010·The New Phytologist·M R BroadleyD E Salt
May 24, 2016·Molecular Plant·Xin-Yuan Huang, David E Salt
May 1, 2010·Annals of Botany·P J White, P H Brown
Jun 2, 2009·Current Opinion in Plant Biology·Ivan Baxter
Feb 14, 2018·G3 : Genes - Genomes - Genetics·Duke PauliMichael A Gore
Jan 30, 2021·Journal of Plant Research·Toshihiro Watanabe, Takayuki Azuma
May 22, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Joshua N CobbSusan R McCouch

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