Rapid clearance of circulating haptoglobin from plasma during acute pulmonary embolism in rats results in HMOX1 up-regulation in peripheral blood leukocytes

Journal of Thrombosis and Haemostasis : JTH
John ZagorskiJeffrey A Kline

Abstract

Acute pulmonary embolism (PE) causes pulmonary hypertension (PH) via several mechanisms including pulmonary vasospasm. We hypothesize that PE with associated PH leads to alterations in plasma protein concentrations indicative of disease severity and prognosis. To identify plasma proteins altered in abundance by PE in rats. Plasma samples were obtained from rats at 2, 6 and 18 h after experimental PE produced with intrajugular injection of polystyrene beads at three different levels of severity (mild, moderate and severe). Total plasma protein was separated using two-dimensional sodium dodecylsulfate-polyacrylamide gel electrophoresis (2D SDS-PAGE) and candidate protein spots altered in expression by PE were identified by mass spectroscopy. Haptoglobin identity and amount was verified by western blot analysis. The PE model produced a dose-dependent increase in right ventricular systolic pressure (RVSP) (mmHg) at 2 h: mild 39+/-1.7, moderate 40+/-1.8 and severe 51+/-1.3 mmHg, coincident with significant increases in free plasma (hemoglobin). Combined 2D SDS-PAGE and Western blot analysis indicated time- and dose-dependant loss of plasma haptoglobin levels in response to acute PE. Haptoglobin (HP) was essentially absent from plasm...Continue Reading

References

Oct 10, 2001·Clinics in Chest Medicine·P F FedulloL J Rubin
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 12, 2002·Nature Medicine·Christopher D ReiterMark T Gladwin
Nov 27, 2002·The European Respiratory Journal·D ChemlaS Brimioulle
Dec 19, 2002·Molecular & Cellular Proteomics : MCP·N Leigh Anderson, Norman G Anderson
Dec 31, 2002·The European Respiratory Journal·S EddahibiS Adnot
Mar 18, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Alan E JonesJeffrey A Kline
Sep 4, 2003·The European Respiratory Journal·P DorfmüllerM Humbert
Nov 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·John ZagorskiJeffrey A Kline
Apr 16, 2004·The European Respiratory Journal·P DartevelleG Simonneau
Jul 27, 2004·Proteomics·Chi Feng TsengSimon J T Mao
Oct 14, 2004·Annals of Medicine·Søren K Moestrup, Holger J Møller
Oct 16, 2004·The New England Journal of Medicine·José R Paño-PardoFrancisco Arnalich
Mar 24, 2005·JAMA : the Journal of the American Medical Association·Ryszard M PlutaEdward H Oldfield
Jul 7, 2005·JAMA : the Journal of the American Medical Association·Claudia R MorrisMark T Gladwin
Apr 8, 2006·Physiological Reviews·Stefan W RyterAugustine M K Choi
Jul 4, 2006·Journal of Molecular and Cellular Cardiology·John A WattsJeffrey A Kline
Nov 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·John ZagorskiJohn A Watts
May 6, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Robert E GersztenLorraine B Ware
Sep 24, 2008·International Journal of Experimental Pathology·John Albert WattsJohn Zagorski
Nov 21, 2008·The New England Journal of Medicine·Mark T Gladwin, Elliott Vichinsky
Jul 2, 2009·Journal of Thrombosis and Haemostasis : JTH·J A KlineM M Hogg
Jul 21, 2009·American Journal of Respiratory Cell and Molecular Biology·Stefan W Ryter, Augustine M K Choi

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Citations

Apr 16, 2013·Emergency Medicine Journal : EMJ·Jeffrey A KlineAlan E Jones
Apr 17, 2012·Journal of Proteomics·Elisabetta GianazzaIngrid Miller
Nov 15, 2011·Pulmonary Pharmacology & Therapeutics·John A WattsJeffrey A Kline
Oct 22, 2011·Thrombosis Research·John A WattsJeffrey A Kline
Mar 15, 2018·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Yun-Xia ZhangChen Wang
Feb 21, 2018·The Journal of Trauma and Acute Care Surgery·Christopher Kalhagen BjerkvigGeir Strandenes
Jun 24, 2011·Journal of Biomolecular Structure & Dynamics·Ra'ed M Al-KhatibRosni Abdullah
May 10, 2019·Pharmaceutical Biology·Zhi-Yao WuYong-Jun Tang

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