PMID: 6606682Dec 16, 1983Paper

Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays

Journal of Immunological Methods
T Mosmann

Abstract

A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation. The assay detects living, but not dead cells and the signal generated is dependent on the degree of activation of the cells. This method can therefore be used to measure cytotoxicity, proliferation or activation. The results can be read on a multiwell scanning spectrophotometer (ELISA reader) and show a high degree of precision. No washing steps are used in the assay. The main advantages of the colorimetric assay are its rapidity and precision, and the lack of any radioisotope. We have used the assay to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.

References

Dec 1, 1977·Cellular Immunology·L B LachmanR E Handschumacher

❮ Previous
Next ❯

Citations

Jan 1, 1996·Journal of Surgical Oncology·S RowleyH K Schackert
Oct 15, 1998·The Journal of Pathology·T OhtaS Ohkuma
May 3, 1996·International Journal of Cancer. Journal International Du Cancer·F PericleJ Y Djeu
Sep 4, 1996·International Journal of Cancer. Journal International Du Cancer·T OhiraN Saijo
Sep 27, 1996·International Journal of Cancer. Journal International Du Cancer·Y TomitaK Takahashi
Nov 15, 1996·International Journal of Cancer. Journal International Du Cancer·T KawasakiT Kumanishi
Nov 15, 1996·International Journal of Cancer. Journal International Du Cancer·Q J WangT E Adrian
Sep 25, 1997·International Journal of Cancer. Journal International Du Cancer·T IshidaN Saijo
Dec 16, 1997·International Journal of Cancer. Journal International Du Cancer·J H BaekK W Kim
Feb 18, 1998·International Journal of Cancer. Journal International Du Cancer·X W Wang, B Xu
Feb 6, 1999·International Journal of Cancer. Journal International Du Cancer·K KitaA Watanabe
Sep 2, 1999·International Journal of Cancer. Journal International Du Cancer·K A SmansJ L Millán
Oct 3, 1999·International Journal of Cancer. Journal International Du Cancer·K KobayashiH Fromm
May 8, 2000·International Journal of Cancer. Journal International Du Cancer·C Y SasakiA Passaniti
Feb 15, 1996·Journal of Neuroscience Research·B S ReinosoP Levitt
Jun 15, 1997·Journal of Neuroscience Research·G RimonL L Rubin
Oct 23, 1997·Journal of Neuroscience Research·R HavivR Stein
Dec 1, 1996·Journal of Biomedical Materials Research·P C LeeC L Tsai
Jan 1, 1996·Journal of Biomedical Materials Research·J C GeesinR A Berg
Sep 5, 1997·Journal of Biomedical Materials Research·P Pellen-MussiM Bonnaure-Mallet
May 13, 1999·Journal of Biomedical Materials Research·C R HowlettD R McKenzie
Sep 17, 1999·Journal of Biomedical Materials Research·A YamamotoM Sumita

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.