Rapid colorimetric detection of p53 protein function using DNA-gold nanoconjugates with applications for drug discovery and cancer diagnostics

Colloids and Surfaces. B, Biointerfaces
Enock AssahYen Nee Tan

Abstract

The tumor suppressor protein p53 plays a central role in preventing cancer through interaction with DNA response elements (REs) to regulate target gene expression in cells. Due to its significance in cancer biology, relentless efforts have been directed toward understanding p53-DNA interactions for the development of cancer therapeutics and diagnostics. In this paper, we report a rapid, label-free and versatile colorimetric assay to detect wildtype p53 DNA-binding function in complex solutions. The assay design is based on a concept that alters interparticle-distances between RE-AuNPs from a crosslinking effect induced through tetramerization of wildtype p53 protein (p53-WT) upon binding to canonical DNA motifs modified on gold nanoparticles (RE-AuNPs). This leads to a visible solution color change from red to blue, which is quantifiable by the UV- visible absorption spectra with a detection limit of 5 nM. Contrastingly, no color change was observed for the binding-deficient p53 mutants and non-specific proteins due to their inability to crosslink RE-AuNPs. Based on this sensing principle, we further demonstrate its utility for fast detection of drug-induced DNA binding function to cancer-associated Y220C mutant p53 protein usi...Continue Reading

Citations

Apr 9, 2020·International Journal of Molecular Sciences·Xue BaiLin Feng
Dec 29, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Haixia WangYu Tao
Feb 9, 2021·Chemical Engineering Journal·Shariq SulemanTejraj M Aminabhavi
Feb 20, 2021·Materials Science for Energy Technologies·Victoria Xin Ting ZhaoXiaodong Zhou

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