Rapid colorimetric detection of Salmonella typhimuriumusing a selective filtration technique combined with antibody-magnetic nanoparticle nanocomposites

Analytical and Bioanalytical Chemistry
Won-Bo ShimMin-Gon Kim

Abstract

Detection of pathogenic bacteria that pose a great risk to human health requires a rapid, convenient, reliable, and sensitive detection method. In this study, we developed a selective filtration method using monoclonal antibody (MAb)-magnetic nanoparticle (MNP) nanocomposites for the rapid and sensitive colorimetric detection of Salmonella typhimurium. The method contains two key steps: the immunomagnetic separation of the bacteria using MAb-MNP nanocomposites and the filtration of the nanocomposite-bound bacteria. Color signals from the nanocomposites remaining on the membrane were measured, which reflected the amount of bacteria in test samples. Immunomagnetic capture efficiencies of 8 to 90 % for various concentrations of the pathogen (2 × 10(4)-2 × 10(1) cells) were obtained. After optimization of the method, 2 × 10(1) cells of S. typhimurium in pure culture solution was detectable as well as in artificially inoculated vegetables (100 cells/g). The method was confirmed to be highly specific to S. typhimurium without cross-reaction to other pathogenic bacteria and could be concluded within 45 min, yielding results in a shorter or similar time period as compared with recently reported antibody immobilized on magnetic-particle...Continue Reading

References

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Citations

Jul 6, 2019·Frontiers in Bioengineering and Biotechnology·Chen XuAiguo Wu
Sep 28, 2017·Biosensors & Bioelectronics·Youngsang YouSundaram Gunasekaran
Mar 14, 2020·Analytical Chemistry·Haihong YangHao Pei

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