Rapid colorimetric lactoferrin-based sandwich immunoassay on cotton swabs for the detection of foodborne pathogenic bacteria

Talanta
Saleh AlamerMohammed Zourob

Abstract

Cotton swab is the conventional swabbing tool that is usually applied for collecting pathogens from contaminated surfaces, followed by cells lysis and DNA extraction before subjecting to genetic analysis. However, such an approach is time consuming as it involves several steps and requires highly trained personnel to perform the experiment. In this study, we developed a new cotton swab-based detection system that involved integrating bacterial collection, preconcentration and detection on Q-tips. The platform is based on a sandwich assay that can detect different pathogens visually by color changes. Lactoferrin-immobilized cotton is used as a general capturing tool to collect various pathogens from surfaces. The presence of particular bacteria is then detected by immersing the cotton in antibodies attached to different colored nanobeads. The target cell is captured between the lactoferrin and specific antibody-conjugated beads which results in certain color development. The effectiveness of this simply fabricated sensor was demonstrated using Salmonella typhimurium, Salmonella enteritidis, Staphylococcus aureus and Campylobacter jejuni. The intensity of the color on the cotton surfaces increased with increasing the concentratio...Continue Reading

Citations

Jul 13, 2019·Critical Reviews in Food Science and Nutrition·Wei WuQingli Yang
Jun 4, 2020·Biosensors·Athmar A AliSalam A Ibrahim
Jan 5, 2021·Biosensors & Bioelectronics·Muhabat Adeola RajiMohammed Zourob
Sep 24, 2020·Analytical Methods : Advancing Methods and Applications·Xiangru BaiJiming Hu
Mar 19, 2021·ACS Infectious Diseases·Carly DeusenberyAnita Shukla
May 4, 2021·Talanta·Elham SheikhzadehMohammed Zourob

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