Rapid comparison of metabolites in humans and rats of different sexes using untargeted UPLC-TOFMS and an in-house software platform

European Journal of Mass Spectrometry
Qiande LiangYue Gao

Abstract

Metabolite differences between sexes have rarely been observed in a global manner, but it has recently been made possible by the advancement in metabolomics techniques. In this study, untargeted ultraperformance liquid chromatography coupled to time-of-flight mass spectrometry and an in-house software platform were used for a rapid comparison of sex differences in urinary metabolites in humans and in urinary and serum metabolites in Sprague Dawley (SD) rats. In addition, the species differences of urinary metabolites between humans and SD rats were also observed. Principle component analysis showed that all the observed metabolite sex differences were more distinct in SD rats than in humans, indicating that the sex differences of human urinary metabolites is small compared with that of SD rats. In SD rats, the observed metabolite sex differences were more distinct in urine than in serum, indicating the importance of urine analysis for metabolomics studies. The species differences in the urinary metabolites of humans and SD rats were much more distinct than any of the observed sex differences. Many sex- and species-related markers were discovered and putatively identified. In both humans and SD rats, steroid metabolites appeared...Continue Reading

References

May 1, 1975·The American Journal of Clinical Nutrition·J M Rivers
Jul 28, 1973·British Medical Journal·M S Margolese, O Janiger
Nov 1, 1969·The Journal of Clinical Investigation·J F Tait, B Little
Jan 1, 1982·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·B ZumoffD K Fukushima
Feb 1, 1995·Biology of Reproduction·M R LuckR A Scholes
Sep 1, 1994·Annals of Clinical Biochemistry·E J LambJ M Burrin
Nov 21, 2000·Annals of Clinical Biochemistry·W ShamimJ W Honour
Feb 28, 2003·General and Comparative Endocrinology·Chadi ToumaRupert Palme
Apr 18, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Anna CzyrakKrzysztof Wedzony
Jun 17, 2003·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Gerrit LesafferRaymond Vanholder
Dec 13, 2003·The Journal of Clinical Endocrinology and Metabolism·Tasoula TsilchorozidouGerard S Conway
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yulan WangJürg Utzinger
Jan 12, 2005·The Journal of Endocrinology·S A CavigelliM K McClintock
Jun 11, 2005·Epilepsia·Rafal M KaminskiMichael A Rogawski
Jan 21, 2006·World Journal of Gastroenterology : WJG·Judit BeneBéla Melegh
Jul 27, 2006·Psychosomatic Medicine·Walid K JerjesAnthony J Cleare
Dec 16, 2006·The Journal of Endocrinology·Karine BlouinAndré Tchernof
Jan 5, 2007·Nucleic Acids Research·David S WishartLori Querengesser
Jun 4, 2008·Biological & Pharmaceutical Bulletin·Sukanya JaroenpornKazuyoshi Taya
Oct 28, 2008·Nucleic Acids Research·David S WishartIan Forsythe
Jun 12, 2009·Journal of Women's Health·Mary V Seeman
Dec 5, 2009·The Journal of Veterinary Medical Science·Tatsuya YAMAMOTOKazuyoshi TAYA
Mar 26, 2010·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Marie-Odile M CheliniRupert Palme
Nov 18, 2010·Journal of the History of Biology·Nathan Q Ha
Nov 19, 2010·Bioanalysis·Ra ScheltemaR Breitling
Feb 8, 2011·Journal of Molecular Biology·Xiaofeng HanMichael A Massiah
Mar 4, 2011·The American Journal of Clinical Nutrition·Wassif S WassifNorman F Taylor
Jul 26, 2011·Journal of the American Society of Nephrology : JASN·Pavel A AronovTimothy W Meyer
Nov 15, 2011·Journal of Analytical Toxicology·Melinda K ShelbyRebecca Heltsley

❮ Previous
Next ❯

Citations

Jan 10, 2017·International Journal of Radiation Biology·Evan L PannkukEvagelia C Laiakis
Jan 11, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Heather A PaulRaylene A Reimer
Jan 23, 2020·International Journal of Molecular Sciences·Marianna CaterinoIlaria Campesi
Oct 17, 2018·Metabolites·Kyle J BurghardtZhengping Yi
Sep 27, 2019·Progress in Neurobiology·Floris ChabrunPascal Reynier

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.