Rapid construction of adeno-associated virus vectors expressing multiple short hairpin RNAs with high antiviral activity against echovirus 30

Oligonucleotides
Diana RotheJens Kurreck

Abstract

RNA interference has proven to be a powerful tool to inhibit viruses. For the prevention of viral escape, multiple short hairpin RNAs (shRNAs) will have to be employed. This article describes a rapid procedure for the generation of shRNA expression cassettes by parallel cloning as well as a simple strategy for the combination of selected units. After delivery of the shRNA expression cassettes with adeno-associated virus vectors, inhibition of echovirus 30 as well as silencing of an important cellular cofactor of virus replication were achieved. The procedure has the potential to be generally applicable for silencing of multiple endogenous targets or viruses.

References

May 1, 1990·Journal of Clinical Microbiology·S L Johnston, C S Siegel
Sep 28, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Marlon R VeldwijkStefan Fruehauf
Nov 5, 2002·Methods : a Companion to Methods in Enzymology·Dirk Grimm
Oct 15, 2003·Journal of Virology·Daniel BodenBharat Ramratnam
Feb 14, 2004·Journal of Virology·Atze T DasBen Berkhout
Jan 27, 2005·Journal of Molecular Biology·Steffen SchubertJens Kurreck
Sep 15, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Aure SaulnierFlorence Colbère-Garapin
Oct 6, 2005·Nature Reviews. Microbiology·J Lindsay WhittonRalph Feuer
Jan 7, 2006·BMC Biotechnology·Glen J McIntyre, Gregory C Fanning
Sep 2, 2006·Nucleic Acids Research·Erich KollerNicholas M Dean
Sep 9, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Olivier ter BrakeBen Berkhout
Nov 21, 2007·Hematology·Dirk Grimm, Mark A Kay
Jan 9, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Olivier ter BrakeBen Berkhout
Jun 13, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Henry FechnerWolfgang Poller
Oct 16, 2008·Clinical Microbiology Reviews·Shyam Daya, Kenneth I Berns
Jan 21, 2009·Angewandte Chemie·Jens Kurreck
Jan 23, 2009·Nature·Haruhiko Siomi, Mikiko C Siomi
Jan 23, 2009·Nature·Martin Jinek, Jennifer A Doudna
Feb 4, 2009·Journal of Virological Methods·Diana RotheJens Kurreck
Oct 3, 2009·Annals of the New York Academy of Sciences·Ben Berkhout
Mar 2, 2010·Antiviral Research·Nick C T SchopmanBen Berkhout
Mar 1, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Olivier Ter BrakeBen Berkhout

❮ Previous
Next ❯

Citations

Jun 21, 2013·Molecular Genetics and Genomics : MGG·Nicoletta Potenza, Aniello Russo

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
fluorescence microscopy
transgenic

Related Concepts

Related Feeds

Cell-Type-Specific Viral Vectors (ASM)

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Cell-Type Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.